Rabbit terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 評價 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔末端補體復合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒（CSB-E15838Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TCC C5b-9含量。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，該產(chǎn)品適用于基礎醫(yī)學研究中補體系統(tǒng)功能評估、炎癥反應機制探索以及自身免疫性疾病模型的建立，例如在腎臟疾病、動脈粥樣硬化或神經(jīng)退行性疾病等領域的實驗設計中，可通過檢測血清或血漿中的C5b - 9水平，揭示補體終末通路激活程度及其病理學關聯(lián)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TCC C5b-9
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	97
	Range %	92-103
1:8	Average %	91
	Range %	86-98

回收率：

The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-109
EDTA plasma (n=4)	97	92-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 3 個評價

樣品類型：血清

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評價：我使用CSB-E15838Rb試劑盒測定正常兔血清OD值，測試結果正常

By 陳老師

樣品類型：血清

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評價：我用CSB-E15838Rb檢測正常兔血清中含量，標準曲線的R2可以做到高達0.9924，標準曲線符合要求，樣品OD值其中之一3.255、3.422，檢測結果準確且完美。

By 王老師

樣品類型：血清

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評價：我用CSB-E15838Rb檢測正常兔血清中β-TG含量，標準曲線的R2可以做到高達0.9978，標準曲線符合要求，樣品OD值其中之一0.250、0.254，可以做到平行，檢測結果準確且完美。

By 張老師