Rabbit β-Thromboglobulin,β-TG ELISA Kit
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中文名稱:兔子β血小板球蛋白/β血栓環(huán)蛋白(β-TG)酶聯(lián)免疫試劑盒
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貨號:CSB-E09285Rb
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:兔子β血小板球蛋白/β血栓環(huán)蛋白(β-TG)酶聯(lián)免疫試劑盒(CSB-E09285Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的β-TG含量。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL,適用于血小板功能評估、血栓形成機(jī)制研究、心血管疾病模型病理過程探索等科研領(lǐng)域。為體外研究β - TG在止血、炎癥反應(yīng)及血管內(nèi)皮損傷中的生物學(xué)作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:beta-Thromboglobulin, beta-TG
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縮寫:β-TG
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種屬:Oryctolagus cuniculus (Rabbit)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:12.5 ng/mL-800 ng/mL
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靈敏度:3.12 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays):CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 102 Range % 97-107 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率:
The recovery of rabbit β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 97 90-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 800 2.801 2.869 2.835 2.748 400 2.484 2.398 2.441 2.354 200 1.716 1.768 1.742 1.655 100 0.944 0.968 0.956 0.869 50 0.454 0.468 0.461 0.374 25 0.256 0.248 0.252 0.165 12.5 0.147 0.151 0.149 0.062 0 0.085 0.089 0.087 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
產(chǎn)品評價
樣品類型:血漿(抗凝劑) 檸檬酸三鈉
樣品信息:其他 兔
稀釋比:沒有稀釋
產(chǎn)品評價: 我用CSB-E09285Rb檢測正常兔血清中β-TG含量,標(biāo)準(zhǔn)曲線的R2可以做到高達(dá)0.9921,標(biāo)準(zhǔn)曲線符合要求,樣品OD值其中之一0.264、0.268,可以做到平行,檢測結(jié)果準(zhǔn)確且完美。
By 王老師
樣品類型:血漿(抗凝劑) 檸檬酸鈉
樣品信息:其他 兔
稀釋比:沒有稀釋
產(chǎn)品評價: 我用CSB-E09285Rb檢測正常兔血清中β-TG含量,標(biāo)準(zhǔn)曲線的R2可以做到大于0.99,樣品OD值符合范圍,檢測結(jié)果準(zhǔn)確且完美。
By 花老師
樣品類型:血清
樣品信息:其他 兔
稀釋比:沒有稀釋
產(chǎn)品評價: 我用CSB-E09285Rb檢測正常兔血清中β-TG含量,標(biāo)準(zhǔn)曲線的R2可以做到高達(dá)0.9997,標(biāo)準(zhǔn)曲線符合要求,樣品OD值符合要求,可以做到平行,檢測結(jié)果準(zhǔn)確且完美。
By 王老師
相關(guān)產(chǎn)品
相關(guān)問答
I would like to redirect another rabbit assay to your assay because it is based on antibodies against the human sequence and not rabbit.
I understand that immunogen information for your kits is not normally revealed however if we are going to redirect another assay to your kit we need to know that the antibodies in your kit are directed against the rabbit sequence. If not and they are against a different species then please reveal that species and how cross reactivity has been confirmed.
About Rabbit beta-Thromboglobulin, pls refer to:
https://www.ncbi.nlm.nih.gov/protein/XP_008266105.1
For this kit CSB-E09285Rb, the standard is 293 cell-derived recombinant protein.
Pls let me know if you have any further questions. Thank you.
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