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  4. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

  • 中文名稱(chēng)：
    大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14100r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶聯(lián)免疫試劑盒（CSB-E14100r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的NT5E/CD73含量。NT5E/CD73是一個(gè)重要靶點(diǎn)，它在腺苷代謝中起關(guān)鍵作用。其高表達(dá)可促進(jìn)腫瘤微環(huán)境免疫抑制。研究顯示，通過(guò)抑制CD73活性阻斷腺苷生成，或能逆轉(zhuǎn)免疫抑制，增強(qiáng)機(jī)體抗腫瘤免疫反應(yīng)，當(dāng)前圍繞它的靶向治療研究是熱門(mén)方向。試劑盒檢測(cè)范圍為0.625 mIU/mL-40 mIU/mL，適用于評(píng)估CD73在腫瘤微環(huán)境、神經(jīng)退行性疾病模型或代謝性疾病研究中的表達(dá)特征，也可用于藥物干預(yù)實(shí)驗(yàn)中的生物標(biāo)志物檢測(cè)。為研究腺苷信號(hào)通路及其相關(guān)疾病機(jī)制提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
  • 縮寫(xiě)：
    NT5E/CD73
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測(cè)范圍：
    0.625 mIU/mL-40 mIU/mL
  • 靈敏度：
    0.156 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-96
    1:2Average %87
    Range %82-95
    1:4Average %90
    Range %85-97
    1:8Average %93
    Range %86-101
  • 回收率：
    The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-101
    EDTA plasma (n=4)9186-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    402.504 2.416 2.460 2.358
    201.933 1.824 1.879 1.777
    101.386 1.283 1.335 1.233
    50.814 0.749 0.782 0.680
    2.50.424 0.397 0.411 0.309
    1.250.228 0.213 0.221 0.119
    0.6250.154 0.145 0.150 0.048
    00.103 0.101 0.102 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    5'-nucleotidase family
  • 數(shù)據(jù)庫(kù)鏈接：