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  4. Rat Alanine aminotransferase(ALT) ELISA Kit

Rat Alanine aminotransferase(ALT) ELISA Kit

  • 中文名稱：
    大鼠丙氨酸氨基轉(zhuǎn)移酶(ALT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13024r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠丙氨酸氨基轉(zhuǎn)移酶(ALT)酶聯(lián)免疫試劑盒（CSB-E13024r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的GPT含量。試劑盒檢測范圍為3.12 mIU/mL-200 mIU/mL，該產(chǎn)品適用于實驗室中肝毒性評估、藥物代謝研究、疾病動物模型建立等場景，例如可通過檢測不同處理條件下肝組織勻漿或血清中的ALT水平變化，評估化學(xué)物質(zhì)、藥物或病理狀態(tài)對肝臟功能的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Gpt ELISA Kit; Aat1 ELISA Kit; Gpt1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; ALT1 ELISA Kit; EC 2.6.1.2 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; GPT 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    3.12 mIU/mL-200 mIU/mL
  • 靈敏度：
    0.78 mIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %98
    Range %91-105
    1:4Average %100
    Range %92-110
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of rat ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9690-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    2002.814 2.911 2.863 2.778
    1002.466 2.355 2.411 2.326
    501.659 1.564 1.612 1.527
    250.853 0.812 0.833 0.748
    12.50.469 0.442 0.456 0.371
    6.250.243 0.226 0.235 0.150
    3.120.149 0.141 0.145 0.060
    00.088 0.082 0.085 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since, we cannot collect more than 150 μL of blood from mice, we can use only 50 μL serum for ELISA. Will it fall within the detectable range ?

A:
We are glad to inform you the following details:
CSB-E13024r,Rat Alanine aminotransferase(ALT) ELISA Kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
Q:

CSB-E13024r
1. In what units, the final data will be represented ? i.e in enzyme activity (IU/L) or
enzyme levels (ng/mg of protein).
2. The reference articles (Article attached) given in the website has mentioned the result
in U/I. What is this unit and how this unit is derived ?
3. Kindly mention the species in which the primary antibody (used in this kit) was raised.

A:
Thanks for your inquiry.
Pls check our answer:
The kit csb-e13024r kit uses two antibodies, mouse monoclonal antibody pre-coated in the plate and goat polyclonal antibody with biotin label. Both of the antibodies are use mIU/mL as the unit.
The kit is quantitatively detected the content of Alanine aminotransferase(ALT).
As for the published reference, pls check this link: https://www.cusabio.com/manage/index.php?action=class&todo=content&m=248&page=1&year=1&code=CSB-E13024r
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • 基因功能參考文獻(xiàn)：
    1. Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
    2. Data suggest that both Alt1 and Alt2 are induced in liver during gluconeogenesis and in diabetes (Goto-Kakizaki rats); in hepatocytes, both Alt1 and Alt2 are induced by glucocorticoid dexamethasone and are suppressed by insulin. PMID: 25865565
    3. Although ALT and GMT activities and TNF-alpha and IL-6 concentrations were higher in male rats histopathological findings have shown slightly more advanced fibrotic and inflammatory changes in female rats. PMID: 17582939
    4. Report the early effects of short-term dexamethasone administration on hepatic and serum alanine aminotransferase in the rat. PMID: 18850354
    5. Protein levels are significantly elevated and correlate with activity, providing the molecular basis for the elevated total serum enzyme activity in liver toxicity. PMID: 19085960

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
  • 組織特異性：
    Liver, heart, skeletal muscle, etc.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:81670

    STRING: 10116.ENSRNOP00000044411

    UniGene: Rn.6318