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  4. Rat Anti-Cyclic Citrullinated Peptide Antibody(Anti-CCP-antibody)ELISA Kit

Rat Anti-Cyclic Citrullinated Peptide Antibody(Anti-CCP-antibody)ELISA Kit

  • 中文名稱：
    大鼠抗環(huán)胍氨酸肽抗體(Anti-CCP-antibody)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13830r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠抗環(huán)胍氨酸肽抗體(Anti-CCP-antibody)酶聯(lián)免疫試劑盒（CSB-E13830r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CCP Ab含量。CCP Ab即抗環(huán)瓜氨酸肽抗體，在類風(fēng)濕關(guān)節(jié)炎研究中是重要靶點。它對類風(fēng)濕關(guān)節(jié)炎診斷特異性高，能較早出現(xiàn)于疾病進程中。其研究機制主要是通過識別瓜氨酸化抗原，觸發(fā)免疫反應(yīng)，參與關(guān)節(jié)炎癥破壞，助力疾病早期診斷與病情監(jiān)測。試劑盒檢測范圍為3.12 IU/mL--200 IU/mL，為類風(fēng)濕性關(guān)節(jié)炎等自身免疫性疾病機制研究提供高效工具；檢測 Anti - CCP 抗體對疾病早期診斷及病程監(jiān)測具有重要科研價值；適用于體外實驗中定量評估血清、血漿等生物樣本中抗體水平；科研人員可通過該產(chǎn)品開展疾病模型中的抗體動態(tài)變化研究、藥物干預(yù)效果評價或探索 CCP 抗體與關(guān)節(jié)炎癥的相關(guān)性，為免疫病理機制解析及新型治療策略開發(fā)提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    CCP Ab
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    3.12 IU/mL--200 IU/mL
  • 靈敏度：
    0.78 IU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 85-105  
    1:2 Average % 97  
    Range % 84-105  
    1:4 Average % 96  
    Range % 92-100  
    1:8 Average % 95  
    Range % 88-105  
  • 回收率：
    The recovery of rat anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 83-96  
    EDTA plasma (n=4) 94 85-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    IU/ml OD1 OD2 Average Corrected  
    200 2.614 2.712 2.663 2.550  
    100 1.995 2.012 2.004 1.891  
    50 1.282 1.267 1.275 1.162  
    25 0.682 0.699 0.691 0.578  
    12.5 0.414 0.422 0.418 0.305  
    6.25 0.257 0.256 0.257 0.144  
    3.12 0.202 0.192 0.197 0.084  
    0 0.110 0.115 0.113 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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