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  4. Rat Apolipoprotein M(APOM) ELISA kit

Rat Apolipoprotein M(APOM) ELISA kit

  • 中文名稱：
    大鼠載脂蛋白M(APOM)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001947RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠載脂蛋白M(APOM)酶聯(lián)免疫試劑盒（CSB-EL001947RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOM含量。APOM為一種載脂蛋白，在脂代謝、炎癥反應(yīng)等生理過程中發(fā)揮重要作用。研究機制主要聚焦于其與高密度脂蛋白（HDL）結(jié)合，參與膽固醇逆向轉(zhuǎn)運等過程，還可能和動脈粥樣硬化、神經(jīng)退行性疾病等發(fā)病機制存在關(guān)聯(lián)，是潛在治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品適用于探索APOM在動脈粥樣硬化、糖尿病并發(fā)癥等病理過程中的生物學(xué)功能，也可用于評估藥物干預(yù)或基因調(diào)控對APOM表達(dá)水平的影響。為研究脂質(zhì)代謝機制、建立心血管疾病動物模型提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ApomApolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit; Protein Px ELISA kit
  • 縮寫：
    APOM
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 96
    Range % 92-100
    1:400 Average % 90
    Range % 85-96
    1:800 Average % 90
    Range % 88-94
    1:1600 Average % 100
    Range % 96-105
  • 回收率：
    The recovery of rat APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-94
    EDTA plasma (n=4) 95 90-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    100 2.454 2.460 2.457 2.319
    50 2.045 2.032 2.039 1.901
    25 1.411 1.482 1.447 1.309
    12.5 0.831 0.901 0.866 0.728
    6.25 0.631 0.625 0.628 0.490
    3.12 0.379 0.408 0.394 0.256
    1.56 0.277 0.272 0.275 0.137
    0 0.135 0.140 0.138 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • 基因功能參考文獻(xiàn)：
    1. Hyperglycemia-induced downregulation of apolipoprotein M expression is not via the hexosamine pathway. PMID: 26377577
    2. ApoM occurrence in urine may be considered to be a biomarker of acute renal injury. PMID: 23953565
    3. ApoM levels and expression in rats rendered hyperglycemic by short-term glucose infusion, was investigated. PMID: 17556016
  • 亞細(xì)胞定位：
    Secreted. Note=Associated with HDL.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 組織特異性：
    Expressed by the liver; secreted in plasma.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:55939

    STRING: 10116.ENSRNOP00000001126

    UniGene: Rn.262