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  4. Rat Aspartate Aminotransferase (AST) ELISA kit

Rat Aspartate Aminotransferase (AST) ELISA kit

  • 中文名稱：
    大鼠天門冬氨酸氨基轉(zhuǎn)移酶(AST)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13023r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠天門冬氨酸氨基轉(zhuǎn)移酶(AST)酶聯(lián)免疫試劑盒（CSB-E13023r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GOT1含量。GOT1是一種重要靶點。它參與細胞內(nèi)氨基酸代謝，在細胞能量生成和生物合成中起關鍵作用。研究發(fā)現(xiàn)其異常表達與多種疾病尤其是腫瘤發(fā)生發(fā)展相關，解析其相關機制可助力開發(fā)以GOT1為靶點的新型治療藥物和策略。試劑盒檢測范圍為3.12 mIU/mL-200 mIU/mL，在肝臟病理機制研究、心肌功能實驗及藥物毒性評估等科研場景中具有重要應用價值；適用于體外實驗中的組織損傷模型評估、生化指標動態(tài)監(jiān)測及相關分子機制探索，為疾病模型構建、藥效學評價等基礎研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Got1Aspartate aminotransferase ELISA kit; cytoplasmic ELISA kit; cAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.3 ELISA kit; Cysteine aminotransferase ELISA kit; cytoplasmic ELISA kit; Cysteine transaminase ELISA kit; cytoplasmic ELISA kit; cCAT ELISA kit; Glutamate oxaloacetate transaminase 1 ELISA kit; Transaminase A ELISA kit
  • 縮寫：
    GOT1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    3.12 mIU/mL-200 mIU/mL
  • 靈敏度：
    0.78 mIU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-92
    1:2Average %104
    Range %100-107
    1:4Average %93
    Range %88-97
    1:8Average %98
    Range %92-103
  • 回收率：
    The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9591-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    2002.094 2.068 2.081 1.918
    1001.276 1.310 1.293 1.130
    500.838 0.784 0.811 0.648
    250.521 0.539 0.530 0.367
    12.50.353 0.334 0.344 0.181
    6.250.300 0.288 0.294 0.131
    3.120.241 0.237 0.239 0.076
    00.164 0.162 0.163 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

相關問答

■ 常見問題解答
Q:

Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since we cannot collect more than 150 μL of blood from mice, we can use only 50 μL serum for ELISA. Will it fall within the detectable range ?

A:
We are glad to inform you the following details:
CSB-E13023r,Rat Aspartate Aminotransferase (AST) ELISA kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
If any further questions,pls let me know.

靶點詳情

  • 功能：
    Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
  • 基因功能參考文獻：
    1. Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, mercaptopyruvate sulfurtransferase (MPST)and cysteine aminotransferase (CAT). PMID: 24611772
    2. In vivo lentiviral gene delivery of GOT minimized lesion volume, whereas GOT knockdown worsened stroke outcomes. Thus, brain tissue GOT emerges as a novel target in managing stroke outcomes. PMID: 21361730
    3. Results suggest that downregulation of the endogenous SO(2)/GOT pathway is likely involved in the pathogenesis of ISO-induced myocardial injury. PMID: 20733562
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 組織特異性：
    Expressed in liver and kidney.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:24401

    STRING: 10116.ENSRNOP00000022309

    UniGene: Rn.5819