Rat Beclin-1(BECN1) ELISA kit
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中文名稱:大鼠自噬蛋白(BECN1)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL002658RA
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠自噬蛋白(BECN1)酶聯(lián)免疫試劑盒(CSB-EL002658RA)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的BECN1含量。BECN1是重要的自噬相關(guān)靶點(diǎn)。其參與自噬起始調(diào)控,在細(xì)胞穩(wěn)態(tài)維持中發(fā)揮關(guān)鍵作用。當(dāng)下研究聚焦于其在疾病發(fā)生發(fā)展中的機(jī)制,如腫瘤的生長(zhǎng)、轉(zhuǎn)移等,有望通過(guò)調(diào)控BECN1為相關(guān)疾病治療提供新策略。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,適用于研究自噬相關(guān)疾病模型中BECN1的蛋白表達(dá)動(dòng)態(tài),如阿爾茨海默病、帕金森病等神經(jīng)系統(tǒng)疾病,或癌癥、代謝綜合征等實(shí)驗(yàn)動(dòng)物模型的機(jī)制探索。滿足基礎(chǔ)科研中對(duì)自噬調(diào)控機(jī)制、藥物干預(yù)效果評(píng)估等研究需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名:Becn1Beclin-1 ELISA kit; Coiled-coil myosin-like BCL2-interacting protein ELISA kit; Protein GT197) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa] ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat BECN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 83-90 1:2 Average % 95 Range % 91-99 1:4 Average % 91 Range % 87-96 1:8 Average % 92 Range % 87-95 -
回收率:
The recovery of rat BECN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 92-98 EDTA plasma (n=4) 90 86-95 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.123 1.982 2.053 1.964 5 1.539 1.523 1.531 1.442 2.5 1.017 1.001 1.009 0.920 1.25 0.691 0.687 0.689 0.600 0.625 0.422 0.412 0.417 0.328 0.312 0.268 0.259 0.264 0.175 0.156 0.166 0.161 0.164 0.075 0 0.091 0.087 0.089 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Costunolide ameliorates rotenone-induced Parkinson's disease in rat model via activation of SIRT-1/p-AMPK cascade and suppression of IL-6/STAT3 axis A Sirwi, NS El-Sayed, DAI Albadawi, AE Koshak,Scientific African,2024
- Early-life bisphenol A exposure causes neuronal pyroptosis in juvenile and adult male rats through the NF-κB/IL-1β/NLRP3/caspase-1 signaling pathway: exploration of age and dose as effective covariates using an in vivo and in silico modeling approach AS Al,Molecular and cellular biochemistry,2024
- Activation of SIRT1/Nrf2/HO-1 and Beclin-1/AMPK/mTOR autophagy pathways by eprosartan ameliorates testicular dysfunction induced by testicular torsion in rats RH Abu,Scientific reports,2024
- The hepatoprotective effect of 4-phenyltetrahydroquinolines on carbon tetrachloride induced hepatotoxicity in rats through autophagy inhibition MH Abdelgalil,Biological research,2024
- Correlations between beclin-1 and transforming growth factor-β1 in letrozole induced polycystic ovary syndrome N Hamdy,/,2024
- Potential Antitumor Activity of Combined Lycopene and Sorafenib against Solid Ehrlich Carcinoma via Targeting Autophagy and Apoptosis and Suppressing Proliferation TA El,Pharmaceuticals,2024
- Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain WJ Song,Toxics,2023
- (R, R)-BD-AcAc2 Mitigates Chronic Colitis in Rats: A Promising Multi-Pronged Approach Modulating Inflammasome Activity, Autophagy, and Pyroptosis S Saber,Pharmaceuticals,2023
- Емпагл?флозин зменшу? периферичну нейропат?ю, пов'язану з цукровим д?абетом 2-го типу: глюкозо-незалежний ефект через передачу сигнал?в AMPK NF Abdelkader,/,2023
- Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling NF Abdelkader,Archives of pharmacal research,2022
- AMPK/mTOR-driven autophagy & Nrf2/HO-1 cascade modulation by amentoflavone ameliorates indomethacin-induced? gastric? ulcer MF Balaha,Biomedicine & Pharmacotherapy,2022
- Combining the HSP90 inhibitor TAS-116 with metformin effectively degrades the NLRP3 and attenuates inflammasome activation in rats: A new management?… AA Shaaban,Biomedicine & pharmacotherapy,2022
- Targeting oxidative stress, apoptosis, and autophagy by galangin mitigates cadmium-induced renal damage: Role of SIRT1/Nrf2 and AMPK/mTOR pathways HH Arab,Life Sci,2022
- Targeting inflammation, autophagy, and apoptosis by troxerutin attenuates methotrexate-induced renal injury in rats HH Arab,International immunopharmacology,2022
- Sitagliptin Protects Cardiac Function by Reducing Nitroxidative Stress and Promoting Autophagy in Zucker Diabetic Fatty (ZDF) Rats Zhou Y.et al,cardiovascular drugs and therapy,2018
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense.; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
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基因功能參考文獻(xiàn):
- Results show decreased SRF levels in the substantia nigra (SN) of rotenone-treated rats that was associated with the loss of tyrosine hydroxylase (TH)-positive neurons. SRF expression was also reduced in rotenone-treated PC12 cells in vitro. In addition, Srf knockdown augmented rotenone-induced toxicity in PC12 cells. In contrast, overexpression of Srf attenuated the cells' sensitivity to rotenone. PMID: 29196028
- Expression of Beclin-1 and LAMP-2 in Rat Hippocampus under Conditions of Simulated Gravity Overload in the Caudocranial Vector PMID: 29797121
- Data suggested that Beclin-1-mediated autophagy impairs the expression and function of cleaved caspase-8 to protect against cadmium-induced activation of apoptosis through Fas/FasL signaling pathway. PMID: 28428545
- that after Caspase 3-mediated Beclin 1 cleavage at 146/149 sites, the N-terminal fragments of Beclin 1 may partially translocate into nuclei in neurons subjected to AD-like injury PMID: 28964771
- Lutein-induced autophagy was mediated by the upregulation of BECN1 in IEC-6 cells. PMID: 28893091
- treatment with an autophagy inhibitor decreased LC3II expression.However, miR-30b overexpression decreased Pi-induced expression of autophagy-related marker genes such as BECN1, ATG5, and LC3b, whereas miR-30b downregulation increased Pi-induced expression of these genes. In addition, we found that miR-30b PMID: 28578340
- These results suggest that EA protects against ischemic stroke through the inhibition of autophagosome formation and autophagy, which is mediated through the mTORC1-ULK complex-Beclin1 pathway PMID: 26647915
- in-vitro genetic depletion of beclin-1 in high glucose treated adult rat cardiomyocytes markedly inhibited the level of autophagy and subsequent apoptotic cell death. PMID: 26386349
- Hypoxia-affected fetal renal development was associated with renal apoptosis and Beclin 1 signaling-mediated autophagy. PMID: 24872334
- Beclin 1 has an essential role as a regulator between autophagy and apoptosis in the setting of neointimal formation PMID: 25238224
- veal a novel function of GA binding protein in the regulation of autophagy via transcriptional activation of the BECN1-PIK3C3 complex PMID: 25046113
- These results suggest that IPC inhibits Beclin 1-dependent excessive autophagy in reperfusion phase and cooperates with anti-apoptosis pathway to diminish the cell death induced by the myocardial I/R injury. PMID: 23392707
- Significantly higher level of Beclin-1 and LC3B-II/LC3B-I ratio (both definitive autophagy biomarkers) was observed in the fibrillated myocardium, versus tissue from the nonfibrillated hearts PMID: 23590156
- Rhes robustly binds the autophagy regulator Beclin-1, decreasing its inhibitory interaction with Bcl-2 independent of JNK-1 signaling. PMID: 24324270
- Level of beclin-1 in acute on chronic liver failure was lower than control. PMID: 24579466
- The results of yhis study found that levels of autophagy markers such as LC3 and Beclin 1 are elevated in the GABAergic interneurons of the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain. PMID: 23665054
- Beclin-1 upregulation results in enhanced levels of autophagy. PMID: 22917477
- beta-asarone can increase Bcl-2 level, finally interfere with the functions of Beclin 1 during the execution of autophagy. PMID: 22306244
- Inhibition of autophagy by Beclin 1 knockdown can attenuate the secondary thalamic damage after focal cerebral infarction. PMID: 22108007
- beclin1 may be involved in the pathogenesis of diabetic nephropathy with the mechanism related to the process of programmed cell death. PMID: 21866636
- Beclin 1-independent autophagy is an important contributor to both the caspase-dependent and -independent components of neuronal apoptosis and may be considered as an important therapeutic target in neural conditions involving apoptosis PMID: 21646862
- Data reveal that Beclin 1 and ATG5 play key roles in morphine-induced autophagy, which may contribute to morphine-induced neuronal injury. PMID: 20190558
- Hypoxia triggers a feedback mechanism that delays apoptosis of oxygen-deprived malignant intestinal epithelial cells and is driven by hypoxia-induced Beclin 1 downregulation. PMID: 19901551
- The inhibition of autophagy by urocortin is mediated in part by inhibition of Beclin1 expression, an effect which is mediated by activation of the PI3 kinase/Akt pathway but which does not involve activation of p42/p44 MAPK. PMID: 16697404
- Beclin 1 protein began to elevate at 1 h time point post-TBI in neurons, 3 d later in astrocytes, and peaked at about 8 d post-TBI. PMID: 18500386
- stimulation of autophagy by -methyl D-aspartate 2B glutamate signaling may be regulated by redistribution of Beclin-1 in membrane rafts PMID: 19335206
- the AMPK-MEK/ERK-TSC-mTOR pathway regulation of Beclin 1 represents different thresholds responsible for a protective or destructive autophagy PMID: 19520853
- Data show that RNA interference-mediated downregulation of Beclin1 improves outcomes after transient middle cerebral artery occlusion. PMID: 19574998
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亞細(xì)胞定位:Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome.; [Beclin-1-C 35 kDa]: Mitochondrion. Nucleus. Cytoplasm.; [Beclin-1-C 37 kDa]: Mitochondrion.
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蛋白家族:Beclin family
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