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Rat C-C motif chemokine 6 (CCL6) ELISA kit

  • 中文名稱:
    大鼠C-C趨化因子6(CCL6)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL027415RA
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠C-C趨化因子6(CCL6)酶聯(lián)免疫試劑盒(CSB-EL027415RA)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CCL6含量。CCL6屬于趨化因子,能趨化特定免疫細(xì)胞到炎癥部位。其研究機(jī)制主要圍繞它與受體相互作用,調(diào)節(jié)免疫細(xì)胞遷移和活化。深入研究CCL6對(duì)揭示免疫調(diào)節(jié)機(jī)制有意義,也有助于探索相關(guān)疾病如炎癥性疾病、腫瘤等的治療靶點(diǎn)。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,可為科研人員研究炎癥性疾病發(fā)生機(jī)制、免疫細(xì)胞趨化行為調(diào)控、感染病理模型中的細(xì)胞因子網(wǎng)絡(luò)變化等課題提供可靠工具,尤其適用于動(dòng)物實(shí)驗(yàn)?zāi)P椭汹吇蜃觿?dòng)態(tài)監(jiān)測(cè)、藥物干預(yù)效果評(píng)估等基礎(chǔ)研究場(chǎng)景。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名:
    Ccl6C-C motif chemokine 6 ELISA Kit; Small-inducible cytokine A6 ELISA Kit
  • 縮寫:
    CCL6
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 94
    Range % 87-98
    1:2 Average % 98
    Range % 91-104
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 97
    Range % 92-103
  • 回收率:
    The recovery of rat CCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 87-96
    EDTA plasma (n=4) 98 93-104
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.634 2.782 2.708 2.604
    5 2.178 2.358 2.268 2.164
    2.5 1.572 1.556 1.564 1.460
    1.25 0.852 0.860 0.856 0.752
    0.625 0.413 0.426 0.420 0.316
    0.312 0.317 0.301 0.309 0.205
    0.156 0.204 0.200 0.202 0.098
    0 0.103 0.105 0.104  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn):
    1. The mouse C10 analog gene in the rat, and named it as ccl6, was identified and cloned. PMID: 23212328
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫(kù)鏈接: