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  4. Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

  • 中文名稱：
    大鼠Ⅰ型膠原C端肽(CTX-Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12776r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠Ⅰ型膠原C端肽(CTX-Ⅰ)酶聯(lián)免疫試劑盒（CSB-E12776r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTX-含量。CTX-Ⅰ是Ⅰ型膠原的降解產(chǎn)物，反映骨吸收活性。在嚴重燒傷或骨質(zhì)疏松等病理狀態(tài)下，CTX-Ⅰ水平升高，提示骨吸收增加。研究顯示，CTX-Ⅰ可作為骨代謝異常的標志物，其機制與破骨細胞活性增加和成骨細胞功能受損有關。試劑盒檢測范圍為25 pg/mL-2000 pg/mL，骨代謝機制研究、骨質(zhì)疏松動物模型評價、骨吸收抑制劑藥效分析、骨相關疾病的基礎研究和藥物開發(fā)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C-telopeptide of type 1 collagen,CTX-1,_x000D_C-telopeptide of type I collagen,CTX-I
  • 縮寫：
    CTX-
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    25 pg/mL-2000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-97  
    1:2 Average % 92  
    Range % 88-96  
    1:4 Average % 98  
    Range % 94-102  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-102  
    EDTA plasma (n=4) 86 82-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.353 0.354 0.354    
    750 0.468 0.475 0.472    
    375 0.616 0.605 0.611    
    125 1.011 0.995 1.003    
    25 1.512 1.594 1.553    
    0 2.013 2.114 2.064    
  • 本試劑盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-human CTX-I antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-rat CTX-I Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated CTX-I 1 x 6 ml -- Bind to the CTX-I antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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產(chǎn)品評價

相關問答

■ 常見問題解答
Q:

I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

A:
Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0；1:10；1:100 is advised.（you can consider use PBS as diluent solution）.
If any further questions,pls do not hesitate to tell me.
Q:

What's the antibody information of this kit?

A:
Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 最新研究進展：
    CTX，全稱為膠原交聯(lián)肽，是一種骨代謝標記物，用于評估骨代謝和骨質(zhì)疏松癥等骨疾病的風險。最新的研究顯示，CTX與骨轉(zhuǎn)換有關，在骨吸收和骨生成的過程中扮演重要的角色。CTX的水平也與肝臟疾病、腎臟疾病、糖尿病和代謝綜合癥等多種疾病有關。近年來，CTX已成為評估骨質(zhì)疏松癥治療效果的重要指標。