Rat Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠Ⅰ型前膠原羧基端肽(PⅠCP)酶聯(lián)免疫試劑盒（CSB-E08081r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PⅠCP含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，可應(yīng)用于骨骼發(fā)育研究、纖維化疾病模型評估、骨代謝相關(guān)藥物篩選等科研領(lǐng)域，為探究骨形成活性、組織修復(fù)機(jī)制及病理狀態(tài)下的膠原合成動態(tài)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
縮寫：

PⅠCP
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	88-97
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-89
EDTA plasma (n=4)	90	85-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.