Rat Carcinoembryonic Antign,CEA ELISA Kit

文獻(xiàn)引用 (2) 說(shuō)明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠癌胚抗原(CEA)酶聯(lián)免疫試劑盒（CSB-E13926r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CEA含量。癌胚抗原（CEA）是一種存在于粘膜細(xì)胞中的糖蛋白，正常情況下在嬰幼兒和成人粘膜細(xì)胞中存在。在癌癥患者中，癌胚抗原的表達(dá)會(huì)增加，因此常作為腫瘤標(biāo)志物。CEA的研究機(jī)制涉及其在細(xì)胞粘附、免疫反應(yīng)、失巢凋亡和肝轉(zhuǎn)移中的作用。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，癌癥機(jī)制研究、實(shí)驗(yàn)動(dòng)物模型中的腫瘤進(jìn)展監(jiān)測(cè)、體外研究腫瘤微環(huán)境、評(píng)估抗癌藥物干預(yù)效果、篩選潛在生物標(biāo)志物、腫瘤生物學(xué)研究、藥理毒理實(shí)驗(yàn)、基礎(chǔ)醫(yī)學(xué)探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

N/A
縮寫：

CEA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	86	80-89

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.