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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rat Caspase-9,Casp-9 ELISA Kit
  • 中文名稱：
    大鼠胱天蛋白酶9(Casp-9)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08863r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胱天蛋白酶9(Casp-9)酶聯(lián)免疫試劑盒（CSB-E08863r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Casp-9含量。Casp-9即半胱天冬酶9，在細(xì)胞凋亡機(jī)制中有重要背景。它是凋亡途徑關(guān)鍵成員，作為凋亡啟動(dòng)者，被細(xì)胞色素C激活后，能招募相關(guān)蛋白形成復(fù)合物，激活下游Caspases級(jí)聯(lián)反應(yīng)，引發(fā)細(xì)胞凋亡，是腫瘤等疾病研究的潛在靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，可廣泛應(yīng)用于基礎(chǔ)科研領(lǐng)域，如凋亡信號(hào)通路機(jī)制研究、藥物開(kāi)發(fā)中的靶點(diǎn)驗(yàn)證或疾病模型中Caspase - 9活性變化的動(dòng)態(tài)監(jiān)測(cè)，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    Casp-9
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-99
    1:2Average %95
    Range %91-102
    1:4Average %98
    Range %94-103
    1:8Average %102
    Range %96-106
  • 回收率：
    The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10096-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40001.846 1.946 1.896 1.752
    20001.442 1.542 1.492 1.348
    10000.986 1.016 1.001 0.857
    5000.546 0.556 0.551 0.407
    2500.320 0.330 0.325 0.181
    1250.266 0.278 0.272 0.128
    62.50.204 0.207 0.206 0.062
    00.143 0.144 0.144 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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