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  4. Rat Catalase (CAT) ELISA kit

Rat Catalase (CAT) ELISA kit

  • 中文名稱：
    大鼠過氧化氫酶(CAT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13439r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠過氧化氫酶(CAT)酶聯(lián)免疫試劑盒（CSB-E13439r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的CAT含量。試劑盒檢測范圍為93.75 mIU/mL-6000 mIU/mL，適用于氧化應(yīng)激機(jī)制研究、藥物毒性評估及炎癥反應(yīng)模型等科研領(lǐng)域，為心血管疾病、神經(jīng)退行性疾病等動物模型中的抗氧化系統(tǒng)功能研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cat ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    93.75 mIU/mL-6000 mIU/mL
  • 靈敏度：
    23.43 mIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 98  
    Range % 94-102  
    1:10 Average % 94  
    Range % 90-97  
    1:20 Average % 105  
    Range % 98-109  
    1:40 Average % 88  
    Range % 82-94  
  • 回收率：
    The recovery of rat CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 82-94  
    EDTA plasma (n=4) 92 87-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    6000 2.703 2.602 2.653 2.537  
    3000 1.962 1.861 1.912 1.796  
    1500 1.218 1.197 1.208 1.092  
    750 0.734 0.723 0.729 0.613  
    375 0.387 0.398 0.393 0.277  
    187.5 0.297 0.286 0.292 0.176  
    93.75 0.188 0.184 0.186 0.070  
    0 0.115 0.117 0.116 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    CAT全稱為過氧化氫酶(Catalase)，是細(xì)胞內(nèi)最重要的抗氧化酶之一。它能將細(xì)胞內(nèi)的過氧化氫分解成水和氧氣，保護(hù)細(xì)胞免受氧化應(yīng)激的損害。最新研究表明，CAT在胰島素分泌、紅細(xì)胞代謝、心血管疾病和神經(jīng)退行性疾病等方面發(fā)揮重要作用。此外，針對CAT的藥物開發(fā)也備受關(guān)注，目前已有一些CAT激動劑進(jìn)入了臨床試驗(yàn)階段，有望成為抗氧化應(yīng)激治療的新藥物。
  • 功能：
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
  • 基因功能參考文獻(xiàn)：
    1. These results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. PMID: 29847079
    2. fenofibrate almost completely abolished GM-induced reactive oxygen species generation, which seemed to be mediated at least in part by the restoration of the expression of PPARalphadependent antioxidant enzymes, including catalase and superoxide dismutase (SOD)-1. PMID: 28290603
    3. These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging. PMID: 27221506
    4. Catalase promoter region (-185 to +52) that contains binding sites for C/EBP-beta showed an augmentation in the methylation level along with a change in methylation pattern of CpG islands in response to PTU treatment PMID: 26459835
    5. Data show that nuclear factor erythroid 2-related factor (Nrf2), superoxide dismutase Sod1 and catalase antioxidant systems are downregulated in isolated arteries and vascular smooth muscle cells from stroke-prone spontaneously hypertensive rats (SHRSP). PMID: 26503970
    6. mitochondria-targeted antioxidant SkQ1 (50 nmol/kg during 5 days) significantly increased the mRNA levels of Nrf2 transcription factor and Nrf2-induced genes encoding antioxidant enzymes SOD1, SOD2, CAT, and GPx4 in rat peripheral blood leukocytes. PMID: 26071777
    7. Targeted mass spectrometry for the analysis of nutritive modulation of catalase and heme oxygenase-1 expression. PMID: 25639505
    8. Decrease in Sirtuin1 (SIRT1) and nuclear factor erythroid 2-related factor (Nrf2) and increase in nuclear factor kappa B (NFkappaB) gene expression in diabetes were associated with a decrease in CAT and GPx mRNA expression. PMID: 24740756
    9. Coronary resistance regulation seems to be more dependent on the hydrogen peroxide that is maintained at low levels by increased catalase activity. PMID: 24669281
    10. Catalase was found to participate in lead-induced alcohol intake and is elevated in blood following lead exposure. PMID: 23808586
    11. The marked increased in alcohol intake that follows an alcohol deprivation period is mediated by acetaldehyde generated in the brain by the action of catalase. PMID: 23527889
    12. Results suggest that the effects of estrogen on the cell-mediated immune responses are mediated through specific estrogen receptors alpha and beta and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). PMID: 23911387
    13. The content of catalase protein in mitochondrial extract increased with aging. PMID: 22683338
    14. Ornithine alpha-ketoglutarate modulates the levels of antioxidants glutathione peroxidase, superoxide dismutase and catalase, as well as lipid peroxidation products in ammonium acetate treated rats PMID: 22905402
    15. The increased expression of antioxidant catalase, specifically in hippocampal neurons, will provide protection from age-related cognitive decline. PMID: 21942371
    16. Ang II inhibited catalase (CAT) and peroxisome proliferator-activated receptor gamma (PPAR gamma) protein and mRNA expressions. PMID: 21295034
    17. Chronic psychosocial isolation attenuated peroxide-metabolizing enzymes, CAT and GPx, and decreased GLR activity. PMID: 20063054
    18. catalase activities in liver and kidney of diabetic male or female rats were significantly lower than in non-diabetic rats but the reduction was more pronounced in diabetic female rats. PMID: 20830981
    19. This protein has been found differentially expressed in the lumbar spinal cord from rats submitted to peripheral lesion during neonatal period. PMID: 20387083
    20. endogenous PGC-1 alpha is a negative regulator of vascular hypertrophy by up-regulating catalase expression and thus reducing reactive oxygen species levels PMID: 19940161
    21. No significant differences in superoxide dismutase and catalase activities, but MDA was decreased by ghrelin on day 10. Evidence for antioxidant properties of ghrelin in rat testis. PMID: 19891630
    22. in addition to transrepression of proinflammatory transcription factors, peroxisome proliferator-activated receptor-gamma may also be modulating catalase expression PMID: 12456800
    23. Levels of Cat mRNA were significantly reduced in congestive heart failure following myocardial infarction. PMID: 14575298
    24. Catalase activities decreased in the liver and heart of rats with hypothyroidism PMID: 14624470
    25. Over-expression catalase genes in primary hippocampal or cortical cultures resulted in increased enzymatic activity of the cognate protein. PMID: 14713308
    26. Changes in post-brain injury/intervention levels of NGF and activity of three major enzymes responsible for ROS scavenging, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), was determined in the hippocampus PMID: 15287906
    27. Medroxyprogesterone acetate and idarubicin both inhibit blood levels of this enzyme PMID: 15372991
    28. generation of H(2)O(2) and expression of CAT are coordinated and indicative of epidermal differentiation as well as of the role of CAT in repairing redox damage PMID: 15734284
    29. influence of gender upon activities of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD) in hypertension PMID: 15773229
    30. catalase and glutathione peroxidase are downregulated in renal insufficiency PMID: 15777843
    31. rats subjected to ovariectomy presented a significant increase in brain Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters PMID: 15869839
    32. Multiple complex formation of C/EBP-beta regulates transcription in the TATA-less catalase promoter. PMID: 16223488
    33. psychological stressor induces a "priming" effect specifically on the CuZnSOD and CAT activity PMID: 16238459
    34. Results suggest that amphetamine exposure is associated with an imbalance between superoxide dismutase and catalase activity in the prefrontal cortex, hippocampus and striatum. PMID: 16770742
    35. Ischemic attack causes a rapid response in hippocampal tissue as well as in the cerebrospinal fluid, represented by an increase in the activity of endogenous antioxidant enzymes SOD and CAT PMID: 17215005
    36. catalase is a major participant in the defense of 36B10 glioma cells against oxidative stress PMID: 17320761
    37. mRNA and protein expressions of superoxide dismutase (SOD) and catalase increase after ischemia (I) and reperfusion (R) of the rat liver. PMID: 17524832
    38. Nimesulide decreased Cat activity significantly in rats subjected to ischemia. PMID: 17564305
    39. observations are compatible with the presence of endogenous heme-containing catalase with an activity of 825 +/- 15 units, which contributes to mitochondrial protection against endogenous or exogenous H2O2 PMID: 17576767
    40. Findings strongly support the assumption that catalase plays a significant role in acetaldehyde formation directly in the rat brain. PMID: 17597213
    41. Even though mRNA expressions of both catalase and Cu-Zn SOD were not changed, the protein levels increased in parallel to activities in the case of another antioxidant, alpha-lipoic acid. PMID: 18008141
    42. Results describe the activity of superoxide dismutase and catalase at different ages following methylphenidate treatment in young rats. PMID: 18049893
    43. Elevated catalase levels in hearts of long-term type 1 diabetic rats signals improved recovery of postischemic cardiac function and reduced reperfusion arrhythmia. PMID: 18430061
    44. Reduced availability of reactive oxygen species by catalase is a key component of neuroprotection against various toxic stimuli PMID: 19302986

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  • 亞細(xì)胞定位：
    Peroxisome.
  • 蛋白家族：
    Catalase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:24248

    STRING: 10116.ENSRNOP00000011230

    UniGene: Rn.3001