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Rat Chordin-like protein 1(CHRDL1) ELISA kit

  • 中文名稱:
    大鼠腱蛋白樣蛋白1(CHRDL1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL005378RA
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠腱蛋白樣蛋白1(CHRDL1)酶聯(lián)免疫試劑盒(CSB-EL005378RA)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的CHRDL1含量。試劑盒檢測(cè)范圍為0.45 ng/mL-30 ng/mL,適用于基礎(chǔ)科研中 CHRDL1 表達(dá)調(diào)控機(jī)制研究、骨骼發(fā)育模型建立、神經(jīng)再生實(shí)驗(yàn)中的蛋白動(dòng)態(tài)分析,或病理狀態(tài)下組織修復(fù)相關(guān)研究。96 孔板規(guī)格設(shè)計(jì)滿足高通量篩選需求本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名:
    Chrdl1 ELISA Kit; Kjr ELISA Kit; Ng1Chordin-like protein 1 ELISA Kit; Kohjirin ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; Ventroptin ELISA Kit
  • 縮寫:
    CHRDL1
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.45 ng/mL-30 ng/mL
  • 靈敏度:
    0.11 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %81-96
    1:2Average %96
    Range %91-104
    1:4Average %97
    Range %92-105
    1:8Average %95
    Range %89-99
  • 回收率:
    The recovery of rat CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-108
    EDTA plasma (n=4)9186-95
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.052 2.011 2.032 1.900
    151.384 1.283 1.334 1.202
    7.50.868 0.847 0.858 0.726
    3.750.501 0.512 0.507 0.375
    1.80.326 0.337 0.332 0.200
    0.90.243 0.254 0.249 0.117
    0.450.198 0.194 0.196 0.064
    00.132 0.131 0.132  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. Plays a role during anterior segment eye development.
  • 基因功能參考文獻(xiàn):
    1. Ng1 gene could promote the proliferation of endogenous neural stem cells and protect injured neurons following spinal cord injury. PMID: 20459000
    2. Neurogenesin-1 contributes to neuronal differentiation of neural stem cells in the adult brain PMID: 14684875
  • 亞細(xì)胞定位:
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接: