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  4. Rat Ciliary Neurotrophic Factor,CNTF ELISA Kit

Rat Ciliary Neurotrophic Factor,CNTF ELISA Kit

  • 中文名稱：
    大鼠睫狀神經(jīng)營養(yǎng)因子(CNTF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04528r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠睫狀神經(jīng)營養(yǎng)因子(CNTF)酶聯(lián)免疫試劑盒（CSB-E04528r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CNTF含量。CNTF即睫狀神經(jīng)營養(yǎng)因子，可促進(jìn)神經(jīng)元存活和分化。在它是神經(jīng)營養(yǎng)因子家族成員。其研究機(jī)制主要圍繞調(diào)節(jié)神經(jīng)細(xì)胞的生長、發(fā)育和功能展開，還參與調(diào)節(jié)能量代謝等過程，在神經(jīng)系統(tǒng)疾病等研究領(lǐng)域受關(guān)注。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于神經(jīng)退行性疾病模型構(gòu)建、神經(jīng)損傷修復(fù)機(jī)制研究、藥物或基因干預(yù)效果評估等科研領(lǐng)域，為探索CNTF在神經(jīng)系統(tǒng)生理病理過程中的動(dòng)態(tài)變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CntfCiliary neurotrophic factor ELISA kit; CNTF ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 92-100
    1:2 Average % 90
    Range % 82-95
    1:4 Average % 103
    Range % 99-110
    1:8 Average % 87
    Range % 82-98
  • 回收率：
    The recovery of rat CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 82-95
    EDTA plasma (n=4) 108 106-113
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.121 1.985 2.053 1.913
    500 1.598 1.543 1.571 1.431
    250 1.223 1.144 1.184 1.044
    125 0.865 0.911 0.888 0.748
    62 0.542 0.513 0.528 0.388
    31.2 0.339 0.331 0.335 0.195
    15.6 0.257 0.255 0.256 0.116
    0 0.142 0.137 0.140 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
  • 基因功能參考文獻(xiàn)：
    1. endogenous SNCA plays a crucial role in spinal neuronal survival, in which the underlying mechanism may be linked to the regulation both apoptotic genes (Caspase7/9) and CNTF. PMID: 27581683
    2. Activation of CNTF/CNTFRalpha signaling pathway by hRheb(S16H) transduction of dopaminergic neurons PMID: 25799580
    3. FGF-2 down-regulated the expression of endogenous CNTF. PMID: 23271288
    4. CNTF induces a decrease in the sodium current and an increase in resting potential as in sodium inversion potential. PMID: 23665214
    5. Hence we can conclude that increase in the expression of ciliary neurotropic factor gene, as a nerve growth factor, following ultrasound radiation, can be considered as the reason of the effect of ultrasound on the rate of injured nerve regeneration. PMID: 23982826
    6. NO signaling induces CNTF expression in astrocytes that favors the beneficial outcomes of reactive astrogliosis in vivo. PMID: 23264628
    7. The data strongly suggested that intracellular ciliary neurotrophic factor may directly modulate proopiomelanocortin gene expression via the activation of ciliary neurotrophic factor receptors in the cell nucleus. PMID: 22146310
    8. The results of this study showed taht increased CNTF and CNTFR complex in the sprouting, metabolically active supraoptic nucleus are related directly to the sprouting response and not the increase in neurosecretory activity. PMID: 22037350
    9. CNTF promotes motor reinnervation of the MCN stump after its end-to-side neurorrhaphy with ulnar nerve and improves functional recovery of the biceps brachii muscle. PMID: 21696588
    10. Sympathetic neurons respond to stretch with an upregulation of VEGF, which is mediated by the NGF/CNTF and TrkA signaling pathway paralleled by HIF-1alpha expression. PMID: 21640078
    11. CNTF induces regeneration of cone outer segments in a rat model of retinal degeneration PMID: 20209167
    12. The CNTF mRNA in the distal stumps of injured spinal cords increased significantly on postoperation days 1-3, then returned to normal level on day 14. PMID: 19626993
    13. activates spinal cord astrocytes, stimulating their production and release of fibroblast growth factor-2, to increase motor neuron survival PMID: 11771938
    14. CNTF promotes the outgrowth of embryonic cranial motor neurons in isolated stage E12 hindbrain explants. PMID: 11932952
    15. CNTF is expressed in the rat pineal gland and eyes during embryonic development and supports the in vitro survival of neonatal sympathetic neurons, which innervate pineal glands immediately after birth. PMID: 11973480
    16. Retinoic acid had no significant effect on the expression of this protein in OLN-93 oligodendrocytes. PMID: 12397370
    17. CNTF affects adipocyte gene expression, and the specific receptor for this cytokine is induced in rodent models of obesity/type II diabetes PMID: 12424252
    18. In olfactory bulb, ciliary neurotrophic factor is localized to ensheathing cell nuclei, cell bodies and axon-enveloping processes; additionally, individual axons of olfactory neurons are CNTF immunoreactive. PMID: 12849744
    19. Our novel findings of increased cardiac CNTF and cardiopulmonary MCP-1 mRNA indicate a role for these factors in the pathogenesis of heart failure. PMID: 12950323
    20. Results show that ciliary neurotrophic factor (CNTF) and its receptor (CNTFRalpha) are differentially regulated in hippocampal neurons and reactive astrocytes following kainic acid injection. PMID: 15179044
    21. CNTF and its receptors are differentially expressed after optic nerve transection and could help delay retinal ganglion cell death in such a stressful environment. PMID: 15193523
    22. data suggest CNTF decreases lean body weight through a combination of appetite inhibition & ubiquitin-proteasome proteolytic pathway activation; findings show that CNTF is at least in part responsible for anorexia & weight loss found with neuronal injury PMID: 15304243
    23. CNTF-CNTFRalpha activates the JAK/STAT pathway leading to enhanced Cx43 expression and intercellular coupling PMID: 15342787
    24. CNTF/CPT-cAMP-induced retinal ganglion cell survival & axonal regeneration are a result of multiple pathway actions, with PKA as an essential component, but these pathways can function in an antagonistic manner under different conditions. PMID: 15574731
    25. CNTF expression is up-regulated from the day following intracerebral hemorrhage, with increased expression observed in brain tissue surrounding the hematoma lesion and white matter structures in association with astroglial proliferation. PMID: 15755520
    26. in skeletal muscle, CNTF can rapidly decrease sodium currents by altering inactivation gating, probably through an intracellular PKC-dependent mechanism that could lead to decreased membrane excitability PMID: 15831538
    27. Ciliary neurotrophic factor prevents acute lipid-induced insulin resistance by attenuating ceramide accumulation and phosphorylation of c-Jun N-terminal kinase in peripheral tissues PMID: 16396984
    28. A notable decrease in ciliary neurotrophic factor (CNTF) was demonstrated by Western blot after ammonia treatment, concurring with the reduction in CNTF mRNA observed in DNA microarrays. PMID: 16483693
    29. Ciliary neural trophic factor may mimic leptin's effects in skeletal muscle [comment] PMID: 16617151
    30. Results indicate that Sox10 is necessary and sufficient for regulating ciliary neurotrophic factor expression in the peripheral nervous system. PMID: 16684879
    31. Three-dimensional reconstructions of CNTF-immunoreactive axonal bulbar projections of these neurons revealed an ordered bilaterally symmetric pattern. PMID: 16999202
    32. The CNTF signaling pathway acts via regulation of nitric oxide production to modulate developmental programmed cell death of postmitotic rod precursor cells. PMID: 17054938
    33. Co-administration of ciliary neurotrophic factor with its soluble receptor protects against neuronal death and enhances neurite outgrowth. PMID: 18086669
    34. Cntf negatively regulates the phototransduction machinery in rod photoreceptors: implications for light-induced photostasis plasticity. PMID: 18188971
    35. Differentiation of TsAM5D chromaffin cells by glial cell derived neurotrophic factor (GDNF) plus CNTF at 39 degrees C become dependent solely on nerve growth factor (NGF) for their survival and neurite outgrowth. PMID: 18293415
    36. CNTF can increase microglial phagocytosis through a calcium-mediated pathway. Our results also suggest that the upregulation of alphav integrin by CNTF could be involved in the increased phagocytotic activity of microglia. PMID: 18401707
    37. Data show that both ciliary neurotrophic factor and fibroblast growth factor-2 are present in regions of elevated oligodendrocyte progenitor cell proliferation and generation after spinal cord injury and may play a role in injury-induced gliogenesis. PMID: 18615534
    38. increased CNTF occurs in response to conditions which induce high levels of phagocytic activity by perivascular cells in the axotomized neurohypophysis PMID: 18805412
    39. CNTF may act as a protective factor against weight gain during hypercaloric diet and could account for individual differences in the susceptibility to obesity. PMID: 18950628
    40. study showed CNTF is up-regulated by ammonia exposure, through mediation of p38 MAPK activation in astrocytes; also observed that SAPK/JNK & Erk1/2 activations in oligodendrocytes & neurons also play indirect roles in CNTF synthesis by astrocytes PMID: 18992343
    41. studies indicate that CNTF can activate microglia and dendritic-like microglia similar to interleukin-6 (IL-6); however, unlike IL-6, CNTF does not stimulate the expected signaling pathways in microglia, nor does it appear to require gp130 PMID: 19267906
    42. CNTF could promote axon regeneration, Schwann cell migration, monocyte infiltration and activation. CNTF might also indirectly promote axonal regeneration by further activating the JAK-STAT3 pathway. PMID: 19289286
    43. Data demonstrate that CNTF is a potent axon growth-promoting factor for mature retinal ganglion cells (RGCs), and that exogenously applied CNTF stimulates RGCs indirectly via a mechanism that depends on astrocyte-derived CNTF. PMID: 19332123
    44. The effect of gp130 stimulation on glutamate-induced excitotoxicity in primary hippocampal neurons PMID: 12150983

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    CNTF family
  • 組織特異性：
    Nervous system.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:25707

    STRING: 10116.ENSRNOP00000016690

    UniGene: Rn.6067