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Rat Cluster of Differentiation 68, CD68 ELISA kit

  • 中文名稱:
    大鼠CD68分子,CD68酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13297r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠CD68分子,CD68酶聯(lián)免疫試劑盒(CSB-E13297r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的CD68含量。CD68是一種在單核細(xì)胞、巨噬細(xì)胞等免疫細(xì)胞上高度表達(dá)的跨膜糖蛋白。它參與細(xì)胞內(nèi)吞、信號(hào)傳導(dǎo)等多種生理過程。當(dāng)前研究聚焦其在炎癥、腫瘤等疾病中的作用機(jī)制,有望為相關(guān)疾病的診斷和治療提供新靶點(diǎn)和思路。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL,適用于多種科研場(chǎng)景,包括巨噬細(xì)胞活化狀態(tài)分析、炎癥性疾病動(dòng)物模型研究(如關(guān)節(jié)炎或動(dòng)脈粥樣硬化)、組織修復(fù)過程中巨噬細(xì)胞浸潤(rùn)評(píng)估等實(shí)驗(yàn)需求。支持科研人員高效開展免疫細(xì)胞功能研究、病理機(jī)制探索及藥物干預(yù)效果評(píng)價(jià)等實(shí)驗(yàn),尤其適用于心血管、腫瘤微環(huán)境及免疫代謝相關(guān)領(lǐng)域的樣本檢測(cè)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD68 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %86-96
    1:2Average %102
    Range %98-105
    1:4Average %91
    Range %87-95
    1:8Average %95
    Range %91-98
  • 回收率:
    The recovery of rat CD68 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-92
    EDTA plasma (n=4)10194-104
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.210 2.188 2.199 2.063
    201.484 1.444 1.464 1.328
    100.971 1.008 0.990 0.854
    50.622 0.645 0.634 0.498
    2.50.378 0.387 0.383 0.247
    1.250.304 0.299 0.302 0.166
    0.6250.222 0.212 0.217 0.081
    00.138 0.134 0.136
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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