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  4. Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

  • 中文名稱：
    大鼠細(xì)胞色素P450 19A1(CYP19A1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006394RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠細(xì)胞色素P450 19A1(CYP19A1)酶聯(lián)免疫試劑盒（CSB-EL006394RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYP19A1含量。CYP19A1是一種編碼芳香化酶的基因，位于細(xì)胞色素P450超家族，主要在肝臟和脂肪組織中表達(dá)。該酶在雌激素的生物合成中起關(guān)鍵作用，其活性異常與多種癌癥的發(fā)生發(fā)展密切相關(guān)。研究顯示，CYP19A1通過調(diào)控脂代謝和免疫應(yīng)答在腫瘤中發(fā)揮作用，具有遺傳多樣性，其突變可能影響雌激素水平和細(xì)胞凋亡。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于體外分析大鼠內(nèi)分泌調(diào)控機(jī)制、卵巢生理病理變化、環(huán)境污染物對激素代謝的影響等研究，為生殖生物學(xué)、毒理學(xué)及激素相關(guān)疾病模型的建立提供定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cyp19a1 ELISA Kit; Arom ELISA Kit; Cyp19Aromatase ELISA Kit; EC 1.14.14.14 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Estrogen synthase ELISA Kit
  • 縮寫：
    CYP19A1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.68 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %101
    Range %95-107
    1:2Average %91
    Range %86-97
    1:4Average %88
    Range %84-92
    1:8Average %95
    Range %90-100
  • 回收率：
    The recovery of rat CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8580-90
    EDTA plasma (n=4)9691-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    12002.452 2.272 2.362 2.237
    6001.705 1.832 1.769 1.644
    3001.286 1.316 1.301 1.176
    1500.829 0.880 0.855 0.730
    750.504 0.469 0.487 0.362
    37.50.301 0.285 0.293 0.168
    18.750.203 0.211 0.207 0.082
    00.127 0.122 0.125
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 亞細(xì)胞定位：
    Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 數(shù)據(jù)庫鏈接：