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Rat Free Testosterone,F-TESTO ELISA Kit

  • 中文名稱：
    大鼠游離睪酮(F-TESTO)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E05097r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠游離睪酮(F-TESTO)酶聯(lián)免疫試劑盒（CSB-E05097r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的F-TESTO含量。試劑盒檢測范圍為0.3 pg/mL-60 pg/mL，該產(chǎn)品可為生殖生物學(xué)研究、環(huán)境內(nèi)分泌干擾物評(píng)估、睪丸功能障礙模型驗(yàn)證以及雄激素相關(guān)藥物作用機(jī)制探索提供可靠數(shù)據(jù)支持，廣泛應(yīng)用于神經(jīng)內(nèi)分泌調(diào)控、性腺衰老、代謝綜合征等動(dòng)物實(shí)驗(yàn)的激素水平檢測，為科研工作者提供標(biāo)準(zhǔn)化的睪酮活性狀態(tài)分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    F-TESTO
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.3 pg/mL-60 pg/mL
  • 靈敏度：
    0.15 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-101  
    1:2 Average % 95  
    Range % 89-99  
    1:4 Average % 94  
    Range % 90-98  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of rat F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-104  
    EDTA plasma (n=4) 94 88-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    60 0.202 0.205 0.204    
    15 0.359 0.342 0.351    
    4.8 0.610 0.623 0.617    
    1.2 1.007 1.012 1.010    
    0.3 1.464 1.457 1.461    
    0 2.439 2.454 2.447    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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