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  4. Rat Galectin-3(LGALS3) ELISA kit

Rat Galectin-3(LGALS3) ELISA kit

  • 中文名稱：
    大鼠半乳糖凝集素-3(LGALS3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL012887RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠半乳糖凝集素-3(LGALS3)酶聯(lián)免疫試劑盒（CSB-EL012887RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LGALS3含量。LGALS3 是一種具有重要生物學功能的靶點。它參與細胞黏附、增殖、凋亡等多種生理過程。在研究機制上，其與腫瘤發(fā)生、發(fā)展和免疫逃逸密切相關(guān)，還涉及纖維化等疾病進程。對它的深入研究有助于開發(fā)新的疾病診斷方法和治療策略。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于探索大鼠模型中纖維化疾病進展、腫瘤發(fā)生機制、代謝綜合征相關(guān)病理變化或免疫調(diào)節(jié)研究，為動物實驗提供可靠的蛋白定量工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LGALS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %84-93
    1:2Average %100
    Range %95-105
    1:4Average %87
    Range %83-90
    1:8Average %96
    Range %93-99
  • 回收率：
    The recovery of rat LGALS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-91
    EDTA plasma (n=4)8783-90
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.771 2.798 2.785 2.621
    502.066 2.098 2.082 1.918
    251.412 1.400 1.406 1.242
    12.50.841 0.868 0.855 0.691
    6.250.597 0.575 0.586 0.422
    3.120.466 0.478 0.472 0.308
    1.560.318 0.336 0.327 0.163
    00.163 0.165 0.164
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 基因功能參考文獻：
    1. Galectin-3 is a crucial factor in inducing pulmonary artery endothelium cell transformation into VSMCs through Jagged1/Notch1 dependent MyoD and SRF expression in this pathological condition PMID: 30463073
    2. that lack of Gal3 worsens subchronic injury after neonatal focal stroke PMID: 27836669
    3. Therapeutic silencing of galectin3 improves cardiomyocyte apoptosis and survival during heart failure. PMID: 29286090
    4. Gal-3 serves as a critical regulator in the pathogenesis of pulmonary arterial hypertension by regulating the proliferation, differentiation, and extracellular matrix deposition synthesis of pulmonary adventitial fibroblast. PMID: 28826890
    5. Gal-3 is overexpressed in aorta and AVs from PO rats. Gal-3 pharmacological inhibition blocks aortic and AV remodeling in experimental PO. Gal-3 could be a new therapeutic approach to delay the progression and the development of aortic remodeling and AV calcification in PO. PMID: 28758988
    6. Gal-3 plays an important role in the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with the Nox4 and Nox4-derived oxidative stress. PMID: 28431936
    7. These findings demonstrate that Gal-3 is required for TGF-beta1-stimulated vascular fibrosis via a STAT3 signaling cascade and that MMP-9 is also involved in TGF-beta1/Gal-3-induced vascular fibrosis. PMID: 27870162
    8. The increase in myocardial Gal-3 expression was associated with cardiac fibrosis and inflammation. PMID: 28360193
    9. galectin-3 expression in the rat brain seems to be regulated by developmental cascades, and functionally and neuroanatomically related brain nuclei constitutively express galectin-3 in adulthood. PMID: 28255782
    10. Data suggest that inhibition of galectin-3 (here, by soluble dietary fiber supplement, modified citrus pectin) prevents initiation/progression of liver cirrhosis by inducing apoptosis in liver stellate cells. PMID: 27010252
    11. Up-regulated galectin-3 signaling might be involved in the pathogenesis in experimental cardiorenal syndrome PMID: 26875907
    12. Findings suggest the involvement of GAL-3 in the glycation and oxidative stress under diabetic conditions and its cytoprotective role in Schwann cells. PMID: 25481849
    13. This study provides a new insight into the molecular mechanisms of HF mediated by PKC-alpha and galectin-3. PMID: 25489662
    14. galectin-3 is activated in microglia and macrophages according to the progression of glioma. PMID: 23179497
    15. Gal-3 is required for inflammatory and fibrotic responses to aldosterone in vascular smooth muscle. PMID: 23117656
    16. Galectin-3 expression is induced in macrophages, particularly ED1+ macrophages, during the course of wound healing. PMID: 21435650
    17. The morphometric analysis showed a significant decrease in the frequency of myelinated axons, myelin turns (lamellae) and g-ratio in the corpus callosum and striatum of Lgals3(-/-) compared with wild-type (WT) mice. PMID: 21566659
    18. Our results identify a cell type-specific control of galectin-3 synthesis by glucocorticoids in lung bronchiolar Clara cells and interstitial macrophages PMID: 21472689
    19. Abnormally lower level of galectin-3 expression and elevated level of rno-let-7d expression were observed in brain of SHR than those in WKY rat. PMID: 20557304
    20. Basal expression and secretion levels of rat gal-3 are higher in glioma cells compared with normal rat primary astrocytes; upregulated interferon-gamma levels in virally infected glioma cells increase gal-3 secretion into the culture media. PMID: 20980634
    21. galectin-3 may be involved in the complex process of kidney regeneration following ischemia/reperfusion injury PMID: 20865664
    22. Inhibition of galectin-3 using RNAi technique can suppress proliferation and induce apoptosis in hepatic stellate cells. PMID: 19785949
    23. MP20 ia a galectin-3 ligand PMID: 11532191
    24. galectin 3 controls proliferation in thyroid cells PMID: 12615069
    25. data suggest complex induction mechanisms of gal-3 expression in neuronally differentiating PC12 cells involving NGF-, but not CNTF- and IL-6-driven (in NGF-primed cells) Ras/MAPK-related signalling pathways PMID: 14622091
    26. Galectin-3 is the major LDN-binding protein in macrophages. Galectin-3 can mediate recognition and phagocytosis of LDN-coated particles by macrophages. PMID: 15265923
    27. An early increase in galectin-3 expression identifies failure-prone hypertrophied hearts. Galectin-3, a macrophage-derived mediator, induces cardiac fibroblast proliferation, collagen deposition, and ventricular dysfunction. PMID: 15520318
    28. Osteoblastic exposure to irreversible advanced glycation endproducts alters their expression and secretion of galectin-3, which could have significant consequences on osteoblast metabolism and thus on bone turnover. PMID: 15646023
    29. Thus galectin-3 may play several important roles in the CCD, including mediating the adaptation of beta-intercalated cells during metabolic acidosis. PMID: 16131647
    30. siRNA knockdown of Galectin-3 inhibited myofibroblast activation after hepatic injury and may therefore provide an alternative therapeutic approach to the prevention and treatment of liver fibrosis PMID: 16549783
    31. Combined proteome-transcriptome-genome and functional analyses identify gal-3 as a candidate gene/protein in Type 1 diabetes. susceptibility PMID: 16600178
    32. galectin-3 promotes K-Ras signaling to both Raf and PI3-K. 3. PMID: 16691442
    33. Galectin-3 is expressed in the neonatal, young, and mature rat intervertebral disc. PMID: 17202886
    34. Galectin-3 was localized to polymorphonuclear neutrophils, microglia, monocytes and macrophages, suggesting an involvement of galectin-3 in the neuroinflammatory processes leading to brain damage in PM. PMID: 17706429
    35. Abundant galectin-3 observed in the area of severe bone destruction may act as a negative regulator for the upregulated osteoclastogenesis accompanying inflammation to prevent excess bone destruction. PMID: 19015643
    36. These results suggest that Gal-3 expressed by activated microglia/infiltrating macrophages and astrocytes in the ischemic brain may play a role in post-ischemic tissue remodeling by enhancing angiogenesis and neurogenesis. PMID: 19573520

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:83781

    STRING: 10116.ENSRNOP00000014216

    UniGene: Rn.764