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  4. Rat Gamma-glutamyltranspeptidase 1(GGT1) ELISA kit

Rat Gamma-glutamyltranspeptidase 1(GGT1) ELISA kit

  • 中文名稱：
    大鼠γ谷氨酰轉(zhuǎn)肽酶1(GGT1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL009394RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠γ谷氨酰轉(zhuǎn)肽酶1(GGT1)酶聯(lián)免疫試劑盒（CSB-EL009394RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GGT1含量。GGT1是一種編碼γ-谷氨酰轉(zhuǎn)肽酶的基因，該酶是一種質(zhì)膜關(guān)聯(lián)的酶，參與谷胱甘肽的代謝。GGT1基因存在多種轉(zhuǎn)錄本和蛋白質(zhì)變體，其功能與糖尿病和其他代謝性疾病有關(guān)。研究顯示，GGT1基因突變可能引起高GGT血癥，但臨床無相關(guān)癥狀。試劑盒檢測(cè)范圍為4.7 mIU/mL-300 mIU/mL，可為肝臟疾病模型研究、藥物肝毒性評(píng)估、膽道功能相關(guān)分子機(jī)制探索等科研項(xiàng)目提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ggt1; Ggt; Glutathione hydrolase 1 proenzyme; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; Leukotriene-C4 hydrolase; CD antigen CD224
  • 縮寫：
    GGT1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    4.7 mIU/mL-300 mIU/mL
  • 靈敏度：
    1.2 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-103  
    1:2 Average % 90  
    Range % 85-94  
    1:4 Average % 92  
    Range % 88-99  
    1:8 Average % 89  
    Range % 84-93  
  • 回收率：
    The recovery of rat GGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    300 2.379 2.311 2.345 2.252  
    150 1.681 1.726 1.704 1.611  
    75 1.205 1.224 1.215 1.122  
    37.5 0.704 0.726 0.715 0.622  
    18.8 0.342 0.317 0.330 0.237  
    9.4 0.210 0.199 0.205 0.112  
    4.7 0.125 0.118 0.122 0.029  
    0 0.090 0.095 0.093    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
  • 基因功能參考文獻(xiàn)：
    1. an extensive ductular reaction in advanced Hepatocellular carcinoma (HCC) and a strong correlation between PCK7 and levels of gammaGT and the poor prognosis and aggressiveness of HCC, is reported. PMID: 27525410
    2. These data demonstrate the important role of endothelial GGT activity in mediating the vasorelaxant effect of S-nitrosoglutathione in rat aorta . PMID: 22984412
    3. The expression of gamma-glutamyltransferase in rat colon carcinoma cells is distinctly regulated during differentiation and oxidative stress PMID: 12030384
    4. Radiation of CC531 colon carcinoma cells blocked cell proliferation, increased cellular size, initiated apoptosis and upregulated GGT activity and protein levels in a dose- and time-related manner. PMID: 16386375
    5. With the co-existence of inflammation and hypoxia in the brain after ischemic stroke, dynamic changes of gammaGT activity reflect evolving changes of endothelial function. PMID: 16973162
    6. Data suggest that extracellular glutathione elicits gamma-glutamyl transpeptidase and ROS-dependent transactivation of tyrosine kinase signaling that upregulates potassium channel activity or expression via redox-mediated mechanisms. PMID: 19419996
    7. The acylation reaction of GGT by gamma-glutamyl anilides can apparently be accelerated by either electron-withdrawing or electron-donating substituents, suggesting a concerted mechanism involving kinetic general acid catalysis of aniline departure. PMID: 11601992

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Gamma-glutamyltransferase family
  • 組織特異性：
    Detected in adult kidney and mammary gland, and in fetal liver.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:116568

    UniGene: Rn.10010