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  4. Rat Gastrin-releasing peptide(GRP) ELISA kit

Rat Gastrin-releasing peptide(GRP) ELISA kit

  • 中文名稱：
    大鼠胃泌素釋放肽(GRP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009940RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胃泌素釋放肽(GRP)酶聯(lián)免疫試劑盒（CSB-EL009940RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GRP含量。GRP即胃泌素釋放肽，是一種神經(jīng)遞質，在多種生理過程中發(fā)揮作用。其與細胞生長、增殖相關，在腫瘤發(fā)生發(fā)展里有重要地位?？蒲芯劢褂谘芯縂RP及其受體的作用機制，以期通過阻斷信號通路來實現(xiàn)腫瘤等疾病的診斷與治療。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，可為神經(jīng)內分泌研究、消化道生理調控實驗以及腫瘤微環(huán)境分析（如小細胞肺癌模型中GRP的高表達研究）提供可靠數(shù)據(jù)支持，配套提供標準品、檢測抗體及優(yōu)化緩沖體系，適用于細胞實驗、動物模型或離體組織樣本的科研級定量檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GrpGastrin-releasing peptide ELISA Kit; GRP) [Cleaved into: Neuromedin-C ELISA Kit; GRP-10)] ELISA Kit
  • 縮寫：
    GRP
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-95
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 97
    Range % 91-103
  • 回收率：
    The recovery of rat GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-98
    EDTA plasma (n=4) 92 88-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.654 2.526 2.590 2.404
    500 2.147 2.048 2.098 1.912
    250 1.446 1.562 1.504 1.318
    125 0.982 0.996 0.989 0.803
    62.5 0.568 0.551 0.560 0.374
    31.2 0.372 0.361 0.367 0.181
    15.6 0.278 0.274 0.276 0.090
    0 0.186 0.185 0.186 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. Contributes to long-term fear memory, but not normal spatial memory. Contributes to the regulation of food intake.
  • 基因功能參考文獻：
    1. These results suggest that androgen surge during male puberty plays an important role in the development and maintenance of the male-specific GRP function in the rat spinal cord. PMID: 26860455
    2. feeding responses evoked by the large molecular form of gastrin releasing peptide (GRP) in the rat PMID: 24993846
    3. all forms of GRP reduce food intake in obese and lean adult Zucker rats similar to Bn in weanling rats. PMID: 24874706
    4. The site of action of rat gastrin releasing peptide, in regulation of meal size and intermeal interval, is most likely the stomach and/or the duodenum. PMID: 24556509
    5. Gastrin-releasing peptide terminals were found in spinal dorsal horn and trigeminal ganglion. PMID: 24254931
    6. GRP regulation of meal size and intermeal interval occurs at different sites in the rat. PMID: 24291388
    7. Gastrin-releasing peptide acts via postsynaptic bombesin type 2 receptors to modulate inward rectifier K+ and TRPV1-like conductances in rat paraventricular thalamic neurons PMID: 23359674
    8. reduction of meal size and prolongation of intermeal interval (IMI) by GRP-29 require vagal and splanchnic nerves, and the duodenum is the major conduit that communicates prolongation of IMI by GRP-29 with the brain PMID: 22210008
    9. With increasing levels of fear, levels of gastrin-releasing peptide (Grp) are increased in medial prefrontal cortex and basolateral amygdala. PMID: 20974156
    10. GRP-29 and GRP-27 reduced the size of the first meal, prolonged intermeal interval and increased satiety ratio, but GRP-10 failed to exhibit similar feeding responses. The data support a role for GRP-29 in the short-term regulation of food intake. PMID: 21055429
    11. These high-voltage electron microscopy findings provide an ultrastructural basis for understanding how the spinal GRP system regulates male sexual behavior. PMID: 19864484
    12. Data suggest that GRP, by acting on GRP receptors but not via CRF receptors, increases 5-HT neuronal activity in the paraventricular nucleus. PMID: 12138009
    13. GRP in the central part of the amygdala inhibits feeding, may decrease the efficiency of eating, and may act as a satiety signal. PMID: 12419521
    14. the increase in gastrin relesing peptide synthesis in the neurosecretory structures of the hypothalamus shoud be regarded as a compensatory reaction in diabetes PMID: 12469881
    15. GRP mRNA was detected in rat kidney from embryonic day 19 to postnatal day 30 by RT-PCR PMID: 15140764
    16. Data suggest that GRP does affect the stress response. GRP alterations selectively affected fear-related responses. PMID: 17335915
    17. Food deprivation and stimulation of the melanocortin system produce opposing changes in GRP gene expression in the PVN; GRP-containing neurons in the PVN may be part of the hypothalamic signaling pathway controlling food intake and energy balance. PMID: 18818295
    18. the spinal GRP system appears to be a stress-vulnerable center for male reproductive functions PMID: 19169356
    19. Results suggest that androgen signaling plays a pivotal role in the regulation of GRP expression in male lumbar spinal cord. PMID: 19359382

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  • 亞細胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle lumen.
  • 蛋白家族：
    Bombesin/neuromedin-B/ranatensin family
  • 數(shù)據(jù)庫鏈接：