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  4. Rat Hemoglobin (Hb) ELISA Kit

Rat Hemoglobin (Hb) ELISA Kit

  • 中文名稱：
    大鼠血紅蛋白酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13036r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠血紅蛋白酶聯(lián)免疫試劑盒（CSB-E13036r）為競爭法ELISA試劑盒，定量檢測血清、血漿、RBC裂解物.樣本中的HBA1含量。HBA1是人類血紅蛋白α鏈的編碼基因，位于16號(hào)染色體上，參與氧氣運(yùn)輸和氣體交換。其研究機(jī)制涉及血紅蛋白結(jié)構(gòu)、功能及其與疾病的關(guān)系，如α地中海貧血。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，適用于科研領(lǐng)域中對(duì)紅細(xì)胞破壞程度評(píng)估、血液代謝研究以及藥物或毒性物質(zhì)引起的溶血效應(yīng)分析，如建立貧血?jiǎng)游锬Ｐ?、監(jiān)測體外溶血實(shí)驗(yàn)或探究血液代用品功效等研究場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Hba1 ELISA Kit; Hba-a1 ELISA Kit; Hemoglobin subunit alpha-1/2 ELISA Kit; Alpha-1/2-globin ELISA Kit; Hemoglobin alpha-1/2 chain ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, lysate for RBC.
  • 檢測范圍：
    0.78 μg/mL-50 μg/mL
  • 靈敏度：
    0.39 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 101    
    Range % 95-107    
    1:10 Average % 93    
    Range % 89-97    
    1:20 Average % 95    
    Range % 90-100    
    1:40 Average % 90    
    Range % 85-97    
  • 回收率：
    The recovery of rat Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 84-93    
    EDTA plasma (n=4) 92 88-96    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    µg/ml OD1 OD2 Average      
    50 0.169 0.176 0.1725      
    25 0.292 0.311 0.3015      
    12.5 0.486 0.483 0.4845      
    6.25 0.819 0.795 0.807      
    3.12 1.373 1.347 1.36      
    1.56 2.013 2.024 2.0185      
    0.78 2.461 2.493 2.477      
    0 2.721 2.652 2.6865      
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in oxygen transport from the lung to the various peripheral tissues.
  • 基因功能參考文獻(xiàn)：
    1. Results report that hemoglobin chains alpha2 nad beta are expressed in mammalian brain neurons and are regulated by a mitochondrial toxin. PMID: 19479992
  • 蛋白家族：
    Globin family
  • 組織特異性：
    Red blood cells.
  • 數(shù)據(jù)庫鏈接：