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  4. Rat Indoleamine 2,3-dioxygenase,IDO ELISA Kit

Rat Indoleamine 2,3-dioxygenase,IDO ELISA Kit

  • 中文名稱：
    大鼠吲哚胺2,3-雙加氧酶(IDO)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13583r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠吲哚胺2,3-雙加氧酶(IDO)酶聯(lián)免疫試劑盒（CSB-E13583r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IDO1含量。IDO1即吲哚胺2,3 -雙加氧酶1，是一種免疫調(diào)節(jié)酶。其背景在于它可通過催化色氨酸代謝影響免疫微環(huán)境。研究機制方面，它能消耗色氨酸造成T細胞增殖受抑、凋亡增加，還可促進調(diào)節(jié)性T細胞生成，從而幫助腫瘤逃避機體免疫監(jiān)視。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，科研人員可利用開展免疫調(diào)控機制研究、藥物干預(yù)效果評估或疾病模型（如腫瘤、感染性疾病或自身免疫異常）中IDO動態(tài)變化的定量分析，為探索IDO相關(guān)分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ido1 ELISA Kit; Ido ELISA Kit; Indo ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; IDO-1 ELISA Kit; EC 1.13.11.52 ELISA Kit; Indoleamine-pyrrole 2,3-dioxygenase ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %81-92
    1:2Average %98
    Range %93-102
    1:4Average %91
    Range %85-97
    1:8Average %95
    Range %91-99
  • 回收率：
    The recovery of rat IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9689-99
  • 標(biāo)準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.864 2.711 2.788 2.693
    10001.852 1.724 1.788 1.693
    5001.076 1.031 1.054 0.959
    2500.652 0.657 0.655 0.560
    1250.361 0.377 0.369 0.274
    62.50.264 0.254 0.259 0.164
    31.250.172 0.178 0.175 0.080
    00.096 0.094 0.095
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
  • 基因功能參考文獻：
    1. Results show the antidepressant effect of infliximab and suggest that its mechanism is partly related to inhibition of indoleamine 2, 3-dioxygenase/3-hydroxyl amino acid oxygenase pathway activation mediated by tumor necrosis factor-alpha in rat brain. PMID: 27366867
    2. this study suggests a link between melatonin, JNK, FoxO1 and IDO1 that acts as a potential balance regulator of tryptophan metabolism, and offers a new approach to treat diseases related to dysregulation of tryptophan metabolism PMID: 28118532
    3. results suggest that M30 is neuroprotective against CORT-induced depression targeting elevated MAO activities that cause oxidative stress and neuroinflammation, resulting in IDO-1 activation, serotonin deficiency and neurodegeneration. PMID: 27870896
    4. IDO expression in NSCs synergistically potentiates the immune suppression activities of NSCs and could be applicable for the development of therapeutic modalities against various neurodegenerative diseases. PMID: 26636969
    5. study demonstrates that IDO gene transfer into engineered lung allograft tissues significantly attenuates acute allograft damage suggesting local therapy with IDO as a strategy to reduce need for systemic immunosuppression and, thereby, its side effects PMID: 26506443
    6. Indoleamine 2,3-dioxygenase is upregulated in the brain of rats with acute pancreatitis. PMID: 25829217
    7. inhibitory effect of locally delivered CTLA4Ig is dependent on IDO activities within the allograft. PMID: 25498102
    8. Transfer of tolerance to naive recipients is dependent upon IDO-mediated immunosuppression. Initiators of IDO activity, CTLA-4 or soluble CTLA-4 did not mediate this tolerogenic process. PMID: 23607691
    9. results suggest IDO gene expression correlates with the severity of acute rejection and IFN-gamma induced IDO-positive DCs may attenuate acute rejection and catalyze local tryptophan metabolism via IDO enzyme expression,leading to tolerance after liver transplantation PMID: 22454246
    10. Ido1 gene knockout or pharmacological inhibition of hippocampal IDO1 activity attenuated both nociceptive and depressive behavior PMID: 22751107
    11. zebularine plus interferon gamma induce IDO1 gene expression related to demethylation events in the IDO1 promoter region and to up-regulation of expression of transcription factors PMID: 22424783
    12. Detection of IDO mRNA in peripheral blood diagnosed acute rejection in the rat model at an earlier stage. PMID: 22172881
    13. Data suggest that during the first phase of infection the IDO-mediated tryptophan depletion has a predominantly antimicrobial effect. PMID: 19602041
    14. NK cells expression is associated with liver allograft acceptance PMID: 19944758
    15. HO-1/IDO cross-regulation modulates apoptosis and proliferation in rat and human breast cancer cells PMID: 16319139
    16. In conclusion, this study demonstrates an age-related decline in Cu/Zn-SOD and IDO activities, the two enzymes responsible for scavenging O2*-. PMID: 16688932
    17. Report immunological role of indoleamine 2,3-dioxygenase in rat liver allograft rejection and tolerance. PMID: 17645734
    18. CD4+T cells accumulate at the heart transplant site and induce graft endothelial cell expression of Indoleamine 2, 3-dioxygenase (IDO) PMID: 17868070
    19. IDO inhibition for 7 days after transplantation reduces survival, but does not cause acute rejection of the liver in an immunotoleramt model. PMID: 18475196
    20. gene delivery using IDO was unsuccessful in prolonging rat liver allograft survival. PMID: 19177450
    21. In vitro, the proliferative ability of T cells or B cells from experimental autoimmune myastenia gravis rats was inhibited when they were cocultured with bone marrow stromal cells. This inhibitory effect was partially dependent on the secretion of IDO PMID: 19283707
    22. Liver-induced long-term acceptance seen in human combined auxiliary liver and kidney transplantation is at least partly mediated by InDO activity. PMID: 19935463

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  • 亞細胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Indoleamine 2,3-dioxygenase family
  • 數(shù)據(jù)庫鏈接：