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  4. Rat Insulin-Like 3 (INSL3) ELISA Kit

Rat Insulin-Like 3 (INSL3) ELISA Kit

  • 中文名稱：
    大鼠胰島素樣因子3(INSL3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15850r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰島素樣因子3(INSL3)酶聯(lián)免疫試劑盒（CSB-E15850r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的INSL3含量。INSL3是一種胰島素樣激素，主要在性腺組織中表達(dá)，與睪丸沉降、生殖細(xì)胞存活、骨代謝調(diào)控相關(guān)。在綿羊中，INSL3基因可能與角的有無有關(guān)，研究發(fā)現(xiàn)其表達(dá)與公羊睪丸和母羊卵巢組織的差異顯著，且存在多個潛在功能位點(diǎn)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可廣泛應(yīng)用于生殖生物學(xué)領(lǐng)域研究，包括睪丸發(fā)育機(jī)制探索、內(nèi)分泌調(diào)控網(wǎng)絡(luò)解析、生殖相關(guān)疾病動物模型評估，以及藥物或環(huán)境因素對性腺功能影響的體外實(shí)驗(yàn)分析。科研人員可通過檢測不同樣本中 INSL3 的動態(tài)變化，深入探究其在雄性生殖健康中的作用機(jī)制，為相關(guān)基礎(chǔ)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Insl3 ELISA Kit; Rlf ELISA Kit; Insulin-like 3 ELISA Kit; Leydig insulin-like peptide ELISA Kit; Ley-I-L ELISA Kit; Relaxin-like factor) [Cleaved into: Insulin-like 3 B chain; Insulin-like 3 A chain] ELISA Kit
  • 縮寫：
    INSL3
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat INSL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 105
    Range % 101-109
    1:2 Average % 97
    Range % 94-99
    1:4 Average % 88
    Range % 83-96
    1:8 Average % 93
    Range % 89-98
  • 回收率：
    The recovery of rat INSL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 99-105
    EDTA plasma (n=4) 92 85-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 1.978 1.944 1.961 1.759
    5 1.436 1.545 1.491 1.289
    2.5 1.021 1.006 1.014 0.812
    1.25 0.665 0.645 0.655 0.453
    0.625 0.423 0.412 0.418 0.216
    0.312 0.312 0.325 0.319 0.117
    0.156 0.255 0.270 0.263 0.061
    0 0.198 0.205 0.202 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
  • 基因功能參考文獻(xiàn)：
    1. The observed postnatal difference in gubernacular malformations between two rate strains is due to different levels of the insl3 receptor differ among strains. PMID: 21633115
    2. data suggest that INSL3 signaling in fetal gubernaculum induces morphogenetic programs in male genitalia; investigation of differences in gene expression regulation by INSL3 in gubernaculum of male and female genitalia PMID: 20631401
    3. Demonstration of INSL3 as the ligand for LGR8 facilitates understanding of the mechanism of testis descent and allows studies on the role of INSL3 in gonadal and other physiological processes PMID: 12114498
    4. Rat leucine-rich repeat-containing G protein-coupled receptor 8 bound INSL3 with high affinity, clearly demonstrating that it is the rat INSL3 receptor PMID: 15956681
    5. INSL3/leucine-rich repeat-containing G protein-coupled receptor 8 signaling may be involved in the genesis and/or developmental maturation of renal glomeruli PMID: 15956754
    6. Prenatal mono-n-butyl phthlate may inhibit the INSL3 gene expression associated with the transabdominal testicular ascent in fetal rats, thereby causing undescended testis in postnatal offspring PMID: 16338306
    7. In utero exposure to flutamide decreases expression levels of Insl3 particularly at 15 days post conception. PMID: 18772241
    8. INSL3 evidently traverses the blood-testis barrier to enter the seminiferous compartment, rete testis, and epididymis in sufficient concentration to be able to address the specific INSL3 receptors (RXFP2) on post-meiotic germ cells and in the epididymis. PMID: 19420383
    9. Prenatal exposure to diaethylstilbestrol can inhibit transabdominal testicular descent via down-regulating the expression of INSL3, which is induced by down-regulating the expression of SF-1. PMID: 19493424

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 組織特異性：
    Expressed in Leydig cells of the testis, and weakly in the theca interna cells of antral follicles and the corpus luteum of the ovary.
  • 數(shù)據(jù)庫鏈接：