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  4. Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

  • 中文名稱：
    大鼠胰島素樣生長因子結(jié)合蛋白1(IGFBP-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07349r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰島素樣生長因子結(jié)合蛋白1(IGFBP-1)酶聯(lián)免疫試劑盒（CSB-E07349r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IGFBP1含量。IGFBP1即胰島素樣生長因子結(jié)合蛋白1，它在調(diào)節(jié)細胞生長、分化和代謝等方面有重要作用。作為一種靶點，研究機制聚焦于其與胰島素樣生長因子的相互作用，以及對下游信號通路影響，以探尋其在腫瘤、代謝疾病等病理過程中的潛在干預(yù)方式。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)科研領(lǐng)域，可用于研究IGFBP-1在大鼠模型中的表達調(diào)控機制，探索其在代謝性疾病、生長發(fā)育異?；蚪M織損傷修復(fù)中的潛在作用，亦可用于體外藥物干預(yù)實驗或疾病模型構(gòu)建中相關(guān)生物標志物的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 80-94  
    1:200 Average % 97  
    Range % 91-105  
    1:400 Average % 88  
    Range % 81-95  
    1:800 Average % 100  
    Range % 95-94  
  • 回收率：
    The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
    EDTA plasma (n=4) 92 87-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.142 2.287 2.215 2.030  
    500 1.882 1.897 1.890 1.705  
    250 1.297 1.324 1.311 1.126  
    125 0.803 0.815 0.809 0.624  
    62.5 0.492 0.506 0.499 0.314  
    31.2 0.360 0.387 0.374 0.189  
    15.6 0.273 0.288 0.281 0.096  
    0 0.184 0.186 0.185 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • 基因功能參考文獻：
    1. In simulated sleep apnea hypopnea syndrome, intermittent hypoxia in young rats does not cause physical growth retardation, but serum IGF-1 and IGFBP-3 levels decreased with the increase of hypoxia and decline of oxygen saturation. PMID: 22805023
    2. up-regulation of IGFBP-1 in oligodendrocytes in multiple sclerosis may serve two functions: (i) regulate IGF-1 actions, (ii) exert IGF-independent effects through its RGD sequence PMID: 20345750
    3. Insulin regulation of insulin-like growth factor-binding protein-1 gene expression PMID: 11784721
    4. regulation of expression by insulin is impaired by presence of hydrogen peroxide PMID: 11942857
    5. IGFBP-1 and IGFBP-3 are present in phosphorylated form in fasted rat skin, which induced inhibition of collagen biosynthesis in cultured fibroblasts PMID: 12670795
    6. IGFBP-1 released during the early steps of liver tissue damage and repair may interact with liver cells and potentiate the sensitivity of IGF-I to mitogenic signals. PMID: 15070850
    7. In this report we demonstrate that in H4IIE-C3 cells, four distinct classes of GSK-3 inhibitor mimic the effect of insulin on a third thymine-rich insulin response element-containing gene, IGFBP-1. PMID: 15350195
    8. Iron, radical oxygen species, and HIF-2 and -3 as well as prolyl hydroxylase pathways play important roles in mediating effects of hypoxia on IGFBP-1 gene expression in liver. PMID: 16166214
    9. insulin inhibition of IGFBP-1 mRNA levels can occur in the absence of the phosphorylation of Foxo1/Foxo3, whereas activation of the mTOR pathway is both necessary and sufficient PMID: 16455781
    10. Activation of the mTOR pathway, without activation of its upstream regulator PI 3-kinase, reduces IGFBP1 expression. PMID: 17032741
    11. Age-related oxidative stress underlies the upregulated JNK activation and IGFBP-1 expression. PMID: 17645865

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  • 亞細胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：