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  4. Rat Insulin-like growth factor binding protein 2,IGFBP-2 ELISA kit

Rat Insulin-like growth factor binding protein 2,IGFBP-2 ELISA kit

  • 中文名稱(chēng)：
    大鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白2(IGFBP-2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07351r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰島素樣生長(zhǎng)因子結(jié)合蛋白2(IGFBP-2)酶聯(lián)免疫試劑盒（CSB-E07351r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IGFBP2含量。IGFBP2（胰島素樣生長(zhǎng)因子結(jié)合蛋白2）是一種高親和力結(jié)合蛋白，在中樞神經(jīng)系統(tǒng)的IGF（胰島素樣生長(zhǎng)因子）調(diào)節(jié)中發(fā)揮重要作用。研究發(fā)現(xiàn)，IGFBP2在膠質(zhì)瘤的發(fā)生、發(fā)展及預(yù)后中具有重要作用，其通過(guò)IGF依賴(lài)/非依賴(lài)通路、β-catenin通路等多種信號(hào)通路參與調(diào)節(jié)膠質(zhì)瘤細(xì)胞的增殖、侵襲、轉(zhuǎn)移及血管生成等生理過(guò)程。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于科研場(chǎng)景中探索IGFBP - 2在糖脂代謝調(diào)控、生長(zhǎng)發(fā)育異常或腫瘤微環(huán)境中的作用機(jī)制，為研究IGF信號(hào)通路及相關(guān)疾病模型提供靈敏可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; BRL-BP ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 107
    Range % 95-111
    1:40 Average % 104
    Range % 95-108
    1:80 Average % 90
    Range % 83-94
    1:160 Average % 97
    Range % 87-103
  • 回收率：
    The recovery of rat IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 82-94
    EDTA plasma (n=4) 101 90-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.631 2.568 2.600 2.439
    20 2.014 1.964 1.989 1.828
    10 1.126 1.083 1.105 0.944
    5 0.589 0.567 0.578 0.417
    2.5 0.347 0.356 0.352 0.191
    1.25 0.266 0.277 0.272 0.111
    0.625 0.219 0.212 0.216 0.055
    0 0.159 0.163 0.161 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 基因功能參考文獻(xiàn)：
    1. Insulin-like growth factor 2 (IGF2) is an important regulator of fetal growth. PMID: 24352370
    2. Abnormal expression of IGFBP-2 correlates with hyperoxia-induced lung injury in neonatal rats. PMID: 18549709
    3. Depolarization induces insulin-like growth factor binding protein-2 expression in vivo via NMDA receptor stimulation. PMID: 11914028
    4. results show that liver growth and cell proliferation in association with insulin-like growth factor-I and insulin-like growth factor binding proteins 1 and 2 levels are affected in utero by fetal undernutrition PMID: 12902319
    5. In proliferating astrocytes, IGFBP-2 may help target insulin-like growth factors (IGFs) to IGF-1 receptors, and IGFBP-2 proteases may play a role in enhancing the availability of IGFs. PMID: 15248291
    6. cathepsin L created a 14-kDa carboxyl IGFBP-2 fragment that corresponded to the sole antigen detected in IEC-18 cell lysates as well as a 16.5-kDa fragment found in the media PMID: 15705658
    7. retinoic acid modulates the secreted IGFBP-2 and -3 during O(2) exposure and inhibits the increase in collagen that occurs during lung injury PMID: 16055936
    8. Growth inhibition, which was supported by the strong induction of p21CIP1/WAF1, was correlated with increased secretion of IGFBP-2 and, unexpectedly, with its increased localization in the nucleus and particularly in the cytoplasm. PMID: 16131350
    9. IGFBP-2 plays a role in the lamellar bone formation process. PMID: 17308996
    10. In conclusion, MMP-9-induced IGFBP2 proteolysis may be regarded as an important post-translational event involved in astrocytoma aggressiveness. These new findings support drug targeting of MMP-9 as an interesting approach in the treatment of PMID: 18563800
    11. A brain region- and cell type-specific responses to estrogen in rat brain, suggesting that Igf signaling may mediate the estrogen function in the hippocampus. PMID: 18946176

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: rno:25662

    STRING: 10116.ENSRNOP00000023068

    UniGene: Rn.6813