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  4. Rat Interferon α,IFN-α ELISA Kit

Rat Interferon α,IFN-α ELISA Kit

  • 中文名稱：
    大鼠α干擾素(IFN-α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08637r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E08637r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IFNA1含量。IFNA1即干擾素α1，是Ⅰ型干擾素家族成員。它具有抗病毒、免疫調(diào)節(jié)等功能。其研究機(jī)制主要是通過(guò)與細(xì)胞表面受體結(jié)合，激活下游信號(hào)通路，誘導(dǎo)多種抗病毒蛋白表達(dá)，從而發(fā)揮對(duì)病毒復(fù)制和傳播的抑制作用，在抗病毒及免疫相關(guān)研究中受關(guān)注。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于體外免疫學(xué)研究、病毒感染模型評(píng)估或藥物干預(yù)實(shí)驗(yàn)中IFN-α表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit
  • 縮寫(xiě)：
    IFN-α
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-91  
    1:2 Average % 92  
    Range % 85-97  
    1:4 Average % 99  
    Range % 94-104  
    1:8 Average % 103  
    Range % 99-108  
  • 回收率：
    The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-96  
    EDTA plasma (n=4) 96 90-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.087 2.099 2.093 1.916  
    1000 1.595 1.574 1.585 1.408  
    500 1.087 1.096 1.092 0.915  
    250 0.672 0.695 0.684 0.507  
    125 0.491 0.457 0.474 0.297  
    62.5 0.376 0.351 0.364 0.187  
    31.25 0.287 0.299 0.293 0.116  
    0 0.175 0.178 0.177 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Does this ELISA detect IFNalpha 1 or 2?

A:
Thanks for your inquiry. It is designed for IFN alpha 1.

靶點(diǎn)詳情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 數(shù)據(jù)庫(kù)鏈接：