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  4. Rat Interleukin 12,IL-12/P40 ELISA KIT

Rat Interleukin 12,IL-12/P40 ELISA KIT

  • 中文名稱：
    大鼠白介素12(IL-12/P40)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07362r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素12(IL-12/P40)酶聯(lián)免疫試劑盒（CSB-E07362r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12/P40含量。IL12/P40是白細(xì)胞介素-12的β亞基，屬于細(xì)胞因子家族，對T細(xì)胞和自然殺傷細(xì)胞有重要作用。其研究機(jī)制涉及與受體的結(jié)合和信號轉(zhuǎn)導(dǎo)，對免疫應(yīng)答和免疫系統(tǒng)穩(wěn)定至關(guān)重要。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域探究感染性疾病、自身免疫疾病或腫瘤微環(huán)境中IL-12/P40的動態(tài)變化，為免疫調(diào)控機(jī)制研究、動物疾病模型評估或藥物干預(yù)效果分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    IL-12/P40
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %96-106
    1:2Average %94
    Range %90-98
    1:4Average %95
    Range %90-99
    1:8Average %89
    Range %82-94
  • 回收率：
    The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-104
    EDTA plasma (n=4)10397-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.329 2.228 2.279 2.077
    10001.748 1.647 1.698 1.496
    5001.152 1.131 1.142 0.940
    2500.794 0.783 0.789 0.587
    1250.506 0.495 0.501 0.299
    62.50.415 0.404 0.410 0.208
    31.250.308 0.303 0.306 0.104
    00.202 0.201 0.202 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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