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  4. Rat Interleukin 16,IL-16 ELISA KIT

Rat Interleukin 16,IL-16 ELISA KIT

  • 中文名稱：
    大鼠白介素16(IL-16)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07452r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素16(IL-16)酶聯(lián)免疫試劑盒（CSB-E07452r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL16含量。IL16作為一個靶點，其背景上是具有免疫調(diào)節(jié)功能的細胞因子。在研究機制方面，它能與CD4受體結(jié)合，參與T淋巴細胞等的活化、增殖與遷移過程，在免疫相關(guān)疾病研究中受關(guān)注，為疾病治療策略開發(fā)提供潛在方向。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于大鼠炎癥機制研究、免疫相關(guān)疾病模型評估及藥物干預效果分析等科研領(lǐng)域；可滿足細胞因子網(wǎng)絡研究、免疫微環(huán)境分析等實驗需求，尤其適用于神經(jīng)科學、免疫學及藥理學領(lǐng)域的體外樣本檢測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-16
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 98  
    Range % 94-102  
    1:4 Average % 103  
    Range % 98-107  
    1:8 Average % 89  
    Range % 84-96  
  • 回收率：
    The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.757 2.656 2.707 2.524  
    1000 2.223 2.122 2.173 1.990  
    500 1.488 1.467 1.478 1.295  
    250 0.937 0.926 0.932 0.749  
    125 0.589 0.578 0.584 0.401  
    62.5 0.425 0.414 0.420 0.237  
    31.25 0.305 0.297 0.301 0.118  
    0 0.183 0.182 0.183 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價