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  • 中文名稱:
    大鼠白介素1β (IL-1β)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08055r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠白介素1β (IL-1β)酶聯(lián)免疫試劑盒(CSB-E08055r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿.樣本中的IL1B含量。IL1B即白介素-1β,是一種重要的促炎細(xì)胞因子。在炎癥反應(yīng)背景下,它參與免疫調(diào)節(jié)過(guò)程。研究機(jī)制上,IL1B可激活下游信號(hào)通路,介導(dǎo)炎癥細(xì)胞的募集與活化,進(jìn)而放大炎癥反應(yīng),與多種炎癥相關(guān)疾病的發(fā)生發(fā)展密切相關(guān)。試劑盒檢測(cè)范圍為0.156 ng/ml-10 ng/ml.,靈敏度為0.039 ng/ml.。適用于實(shí)驗(yàn)室炎癥模型構(gòu)建、免疫調(diào)控機(jī)制研究、藥物抗炎效果評(píng)估等科研場(chǎng)景,通過(guò)定量分析不同處理?xiàng)l件下IL-1β的動(dòng)態(tài)變化,為炎癥相關(guān)疾病的基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates.
  • 檢測(cè)范圍:
    0.156 ng/ml-10 ng/ml.
  • 靈敏度:
    0.039 ng/ml.
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    88

    Range %

    80-96

    1:2

    Average %

    92

    Range %

    90-105

    1:4

    Average %

    94

    Range %

    91-103

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    99

    90-105

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.903

    2.834

    2.868

    2.689

    5

    2.208

    2.167

    2.187

    2.008

    2.5

    1.563

    1.508

    1.536

    1.356

    1.25

    0.872

    0.859

    0.865

    0.686

    0.625

    0.579

    0.551

    0.565

    0.386

    0.312

    0.363

    0.353

    0.358

    0.178

    0.156

    0.284

    0.274

    0.279

    0.099

    0

    0.184

    0.175

    0.180

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型:組織勻漿液 心

樣品信息:大鼠

稀釋比:沒(méi)有稀釋

產(chǎn)品評(píng)價(jià): 該試劑盒操作簡(jiǎn)便,檢測(cè)快速準(zhǔn)確。試劑性能穩(wěn)定,能滿足不同場(chǎng)景的檢測(cè)需求。包裝嚴(yán)實(shí),標(biāo)識(shí)清晰。價(jià)格合理,性價(jià)比高。無(wú)論是專業(yè)機(jī)構(gòu)還是個(gè)人使用,都能提供可靠的檢測(cè)結(jié)果,為健康保駕護(hù)航。

By 徐老師

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    IL-1β是一種由單核細(xì)胞和巨噬細(xì)胞產(chǎn)生的細(xì)胞因子,能夠介導(dǎo)炎癥和免疫反應(yīng)。最新研究發(fā)現(xiàn),IL-1β參與多種疾病的發(fā)生和發(fā)展,包括腫瘤、自身免疫病和神經(jīng)退行性疾病等。目前已有研究在探索針對(duì)IL-1β的治療方案,如使用IL-1β抑制劑等。此外,一些研究還發(fā)現(xiàn)IL-1β的多態(tài)性可能與特定疾病的風(fēng)險(xiǎn)相關(guān)。
  • 功能:
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亞細(xì)胞定位:
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族:
    IL-1 family
  • 數(shù)據(jù)庫(kù)鏈接: