Rat Interleukin 22(IL-22) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠白介素22(IL-22)酶聯(lián)免疫試劑盒（CSB-E14918r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL-22含量。IL-22是一個(gè)重要靶點(diǎn)，它為白細(xì)胞介素家族成員，與免疫調(diào)節(jié)密切相關(guān)。其研究機(jī)制涉及參與調(diào)節(jié)組織修復(fù)、炎癥反應(yīng)等過程。在多種疾病如自身免疫病、炎癥性腸病等的發(fā)生發(fā)展中發(fā)揮作用，是藥物研發(fā)的潛在方向。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域?qū)L - 22表達(dá)水平的精準(zhǔn)分析；可廣泛用于炎癥性疾病動(dòng)物模型（如結(jié)腸炎、皮炎或呼吸道感染）的病理機(jī)制研究、藥物干預(yù)效果評(píng)價(jià)或免疫調(diào)控實(shí)驗(yàn)本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-22
Uniprot No.：

G3V6X6
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-94
1:2	Average %	99
	Range %	90-102
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of rat IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	104	92-109

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.