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  4. Rat Interleukin-34(IL34) ELISA kit

Rat Interleukin-34(IL34) ELISA kit

  • 中文名稱：
    大鼠白介素-34(IL34)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011657RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素-34(IL34)酶聯(lián)免疫試劑盒（CSB-EL011657RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL34含量。IL34是一個重要靶點。它是一種細胞因子，能與受體結(jié)合激活相關(guān)信號通路。研究發(fā)現(xiàn)其在免疫調(diào)節(jié)、炎癥反應(yīng)等過程中有重要作用，還和多種疾病的發(fā)生發(fā)展相關(guān)。對IL34作用機制的研究，有助于開發(fā)新的疾病治療策略與藥物。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于基礎(chǔ)科研中探索炎癥模型、自身免疫性疾病機制或藥物干預(yù)效果評估，支持科研人員通過多類型生物樣本（如血液或組織提取物）開展系統(tǒng)性研究，為深入解析IL - 34在病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Il34Interleukin-34 ELISA Kit; IL-34 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-93
    1:2 Average % 107
    Range % 103-110
    1:4 Average % 85
    Range % 81-89
    1:8 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 107 103-110
    EDTA plasma (n=4) 86 83-89
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.100 2.168 2.134 1.995
    800 1.654 1.604 1.629 1.490
    400 1.181 1.204 1.193 1.054
    200 0.765 0.778 0.772 0.633
    100 0.517 0.502 0.510 0.371
    50 0.346 0.351 0.349 0.210
    25 0.228 0.231 0.230 0.091
    0 0.140 0.138 0.139 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
  • 基因功能參考文獻：
    1. In a rat cardiac allograft model, IL-34 induced transplant tolerance associated with a total inhibition of alloantibody production. Treatment of rats with IL-34 promoted allograft tolerance mediated by induction of CD8+ and CD4+ Tregs. PMID: 26389674
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-34 family
  • 數(shù)據(jù)庫鏈接：