Rat Ischemia Modified Albumin,IMA ELISA Kit
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中文名稱:大鼠缺血修飾白蛋白(IMA)酶聯(lián)免疫試劑盒
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貨號:CSB-E13620r
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠缺血修飾白蛋白(IMA)酶聯(lián)免疫試劑盒(CSB-E13620r)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IMA含量。大鼠缺血修飾白蛋白(IMA)是心肌缺血早期標(biāo)志物,其形成與缺血時(shí)白蛋白與金屬離子結(jié)合能力改變有關(guān)。IMA檢測可用于急性冠脈綜合征(ACS)的早期診斷和危險(xiǎn)分層,對心肌缺血再灌注治療有指導(dǎo)意義。試劑盒檢測范圍為0.9 U/mL-60 U/mL,適用于大鼠心血管疾病機(jī)制研究、缺血再灌注損傷模型評估、藥物干預(yù)效果分析等科研場景本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:IMA
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.9 U/mL-60 U/mL
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靈敏度:0.225 U/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Others
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat IMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 90-95 1:2 Average % 96 Range % 90-103 1:4 Average % 102 Range % 98-106 1:8 Average % 87 Range % 82-92 -
回收率:
The recovery of rat IMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-97 EDTA plasma (n=4) 89 85-93 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. U/ml OD1 OD2 Average Corrected 60 2.599 2.735 2.667 2.476 30 1.763 1.859 1.811 1.620 15 1.174 1.164 1.169 0.978 7.5 0.658 0.710 0.684 0.493 3.75 0.532 0.539 0.536 0.345 1.8 0.321 0.328 0.325 0.134 0.9 0.245 0.234 0.240 0.049 0 0.191 0.190 0.191 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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