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  4. Rat KI-67 ELISA kit
  • 中文名稱：
    大鼠KI-67酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13175r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠KI-67酶聯(lián)免疫試劑盒（CSB-E13175r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的KI-67含量。KI-67 是一種與細(xì)胞增殖相關(guān)的核抗原。其表達(dá)水平與細(xì)胞增殖活性密切相關(guān)，在正常組織和腫瘤組織中有不同表現(xiàn)。研究中常將其作為評(píng)估細(xì)胞增殖狀態(tài)的重要指標(biāo)，在腫瘤診斷、預(yù)后判斷等方面有重要價(jià)值，用于指導(dǎo)治療方案的制定。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，腫瘤生長(zhǎng)評(píng)估、組織再生研究、體外培養(yǎng)細(xì)胞增殖分析、腫瘤模型研究、藥物作用機(jī)制探索、基礎(chǔ)研究中對(duì)細(xì)胞動(dòng)態(tài)增殖行為的定量化分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Nifk ELISA Kit; Mki67ipMKI67 FHA domain-interacting nucleolar phosphoprotein ELISA Kit; Nucleolar protein interacting with the FHA domain of pKI-67 ELISA Kit
  • 縮寫：
    KI-67
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.04 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat KI-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %83-90
    1:2Average %93
    Range %90-96
    1:4Average %89
    Range %85-93
    1:8Average %100
    Range %95-105
  • 回收率：
    The recovery of rat KI-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9288-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.796 2.731 2.764 2.566
    52.447 2.407 2.427 2.229
    2.51.714 1.768 1.741 1.543
    1.251.029 1.068 1.049 0.851
    0.6250.614 0.638 0.626 0.428
    0.3120.473 0.498 0.486 0.288
    0.1560.331 0.343 0.337 0.139
    00.197 0.199 0.198
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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