Rat Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠細(xì)胞角蛋白18片段Asp396(K18-Asp396)酶聯(lián)免疫試劑盒（CSB-E13708r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CK 18M30含量。試劑盒檢測(cè)范圍為1.56 mIU/mL-100 mIU/mL，適用于基礎(chǔ)科研中動(dòng)態(tài)監(jiān)測(cè)細(xì)胞凋亡水平、評(píng)估藥物干預(yù)效果或探究疾病模型中上皮細(xì)胞損傷機(jī)制。適用于肝臟疾病模型研究、腫瘤生物學(xué)實(shí)驗(yàn)或毒理學(xué)評(píng)估等非臨床科研場(chǎng)景，為細(xì)胞死亡相關(guān)分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

N/A
縮寫：

CK 18M30
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 mIU/mL-100 mIU/mL
靈敏度：

0.39 mIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
Range %	91-102
1:2	Average %	94
Range %	90-98
1:4	Average %	84
Range %	80-88
1:8	Average %	103
Range %	99-112

回收率：

The recovery of rat CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	89-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.