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  4. Rat Klotho ELISA Kit
  • 中文名稱：
    大鼠 Klotho酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14958r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠 Klotho酶聯(lián)免疫試劑盒（CSB-E14958r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KL含量。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于基礎(chǔ)科研中探索Klotho在衰老機(jī)制、腎臟疾病模型、代謝綜合征或藥物干預(yù)研究中的表達(dá)動(dòng)態(tài)，例如通過檢測不同生理狀態(tài)或?qū)嶒?yàn)處理后動(dòng)物血清、組織中的Klotho濃度變化，為相關(guān)分子機(jī)制研究及潛在生物標(biāo)志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    KlKlotho ELISA Kit; EC 3.2.1.31) [Cleaved into: Klotho peptide] ELISA Kit
  • 縮寫：
    KL
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Developmental Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    108

    Range %

    103-114

    1:2

    Average %

    92

    Range %

    88-97

    1:4

    Average %

    103

    Range %

    98-107

    1:8

    Average %

    93

    Range %

    88-98

  • 回收率：

    The recovery of rat klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    90-100

    EDTA plasma (n=4)

    90

    83-97

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    8000

    2.678

    2.634

    2.656

    2.495

    4000

    2.231

    2.107

    2.169

    2.008

    2000

    1.602

    1.578

    1.590

    1.429

    1000

    1.006

    1.029

    1.018

    0.857

    500

    0.658

    0.628

    0.643

    0.482

    250

    0.429

    0.433

    0.431

    0.270

    125

    0.308

    0.294

    0.301

    0.140

    0

    0.157

    0.165

    0.161

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問答

■ 常見問題解答
Q:

I would like to know which type of klotho is determined by this kit (Full length transmembrane protein OR secreted klotho)?

A:
Thanks for your inquiry.
CSB-E14958r
No matter whether klotho exists in membrane form or secreted form, this kit detects klotho in soluble form. Even if it is membrane form, it can still be detected out as long as we change or close the hydrophobic domain of transmembrane region through corresponding method such as grinding and centrifuging homogenates. Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • 基因功能參考文獻(xiàn)：
    1. Intermedin1-53 may attenuate vascular calcification by upregulating alpha-Klotho via the calcitonin receptor/modifying protein complex and protein kinase A signaling. PMID: 26880455
    2. The antiaging protein klotho inhibits angiotensin II -induced cardiomyocyte hypertrophy. PMID: 26970306
    3. The results suggested that the cardioprotective effects of klotho were related to the attenuation of oxidative stress, and oxidative stress suppressed the transcription of klotho PMID: 26093264
    4. klotho is involved in the maintenance of baroreflex sensitivity in the brain. PMID: 24867139
    5. Klotho gene delivery abolished the upregulation of endothelin-1 levels and the downregulation of ETB and Mn-SOD expression in kidneys of spontaneously hypertensive rats PMID: 24979306
    6. Klotho attenuates high glucose-induced fibronectin expression and cell hypertrophy via the ERK1/2 and p38 kinase-dependent pathways. PMID: 24721634
    7. Klotho protects the kidney against cisplatin nephrotoxicity by reduction of basolateral uptake of cisplatin by OCT2 and a direct anti-apoptotic effect independent of cisplatin uptake. PMID: 24304882
    8. These results provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development. PMID: 23838326
    9. Silencing of brain klotho potentiated and expedited cold-induced elevation of blood pressure by upregulation of endothelin-1 and the subsequent activation of the sympathetic nervous system. PMID: 20086041
    10. the therapeutic potential of soluble Klotho protein to treat age-related disorders in mice PMID: 23041151
    11. These results suggest that the expression of Klotho is regulated by the balance between upregulation and downregulation of Wnt signaling. PMID: 22551380
    12. The Klotho gene can attenuate the progression of hypertension and abolishes myocardial hypertrophy and myocardial fibrosis. PMID: 23225045
    13. these studies provide novel evidence for Klotho as a key player in myelin biology, which may thus be a useful therapeutic target in efforts to protect brain myelin against age-dependent changes and promote repair in multiple sclerosis. PMID: 23365232
    14. Aging-related kidney damage is associated with a decrease in klotho expression PMID: 20830528
    15. Renal mRNA and protein expression of Klotho plays important an role in progression of hypertensive renal damage in spontaneously hypertensive rats. PMID: 21646815
    16. Fosinopril up-regulates klotho mRNA in time-dependent manner, and inhibits the down-regulated expression of klotho gene induced by Ang II. PMID: 21038689
    17. Fosinopril and losartan can exert protection against hypertensive renal damage through upregulating Klotho expression as well as reducing oxidative stress. PMID: 21311136
    18. New insights into the pathology of age-related degeneration of dopaminergic neurons are possibly related to Klotho-mediated regulation of vitamin D. PMID: 21276773
    19. Valsartan can improve the brain ultrastructure of spontaneously hypertensive rats by increasing the expression of Klotho. PMID: 20360647
    20. Suggest that klotho may act as a positive regulator of parathyroid hormone expression and secretion in secondary hyperparathyroidism. PMID: 20631679
    21. Decrease of oxidative stress is not only responsible for the improvement of renal function but also for the recovery of Klotho expression in kidney of streptozotocin-induced diabetic rats. PMID: 20625492
    22. AST-120 increases Klotho expression, and inhibited cell senescence in the kidney of CRF rats, probably by alleviating indoxyl sulfate overload on the kidney PMID: 19955715
    23. klotho distribution & expression in rat models of insulin resistance; no significant differences between insulin resistant and control animals; conclude that decreased klotho expression is not a general feature of rodent models of insulin resistance PMID: 19756714
    24. Klotho is post-translationally processed and there are regulatory mechanisms for its secretion in vivo PMID: 15135068
    25. Insulin enhances KL shedding without increasing ADAM10 and ADAM17 mRNA and protein levels PMID: 18056631
    26. Klotho may have a broader function in the regulation of ion transport in the kidney. PMID: 19349416
    27. Klotho may have a role in the modulation of endothelial inflammation. PMID: 19367378

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.; [Klotho peptide]: Secreted.
  • 蛋白家族：
    Glycosyl hydrolase 1 family, Klotho subfamily
  • 組織特異性：
    Present in cortical renal tubules and the parathyroid (at protein level). Strongly expressed in kidney. Expressed at low levels in brain, lung, intestine and ovaries.
  • 數(shù)據(jù)庫鏈接：