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  4. Rat Leukotriene B4,LT-B4 ELISA Kit

Rat Leukotriene B4,LT-B4 ELISA Kit

  • 中文名稱：
    大鼠白三烯B4(LTB4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08035r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白三烯B4(LTB4)酶聯(lián)免疫試劑盒（CSB-E08035r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LT-B4含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于實(shí)驗(yàn)室環(huán)境中開展炎癥相關(guān)疾病模型研究、藥物干預(yù)效果評估或免疫調(diào)控機(jī)制探索等科研場景，尤其適合同步分析血液樣本與組織局部炎癥介質(zhì)的表達(dá)水平，為深入解析LTB4在病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    LT-B4
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %89-99
    1:2Average %97
    Range %91-103
    1:4Average %97
    Range %92-104
    1:8Average %93
    Range %88-97
  • 回收率：
    The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-96
    EDTA plasma (n=4)9793-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.442 2.541 2.492 2.320
    5001.887 1.986 1.937 1.765
    2501.282 1.263 1.273 1.101
    1250.830 0.821 0.826 0.654
    62.50.511 0.502 0.507 0.335
    31.20.370 0.361 0.366 0.194
    15.60.264 0.259 0.262 0.090
    00.171 0.172 0.172 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)