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  4. Rat Mast Cell Tryptase,MCT ELISA Kit

Rat Mast Cell Tryptase,MCT ELISA Kit

  • 中文名稱：
    大鼠肥大細胞類胰蛋白酶(MCT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13627r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠肥大細胞類胰蛋白酶(MCT)酶聯(lián)免疫試劑盒（CSB-E13627r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的TPSAB1含量。TPSAB1，也稱為類胰蛋白酶α/β 1，是一種胰蛋白酶樣絲氨酸蛋白酶，屬于肽酶家族S1。它在肝素穩(wěn)定的四聚體中具有酶促活性，對內(nèi)源性蛋白酶抑制劑有抗性。TPSAB1基因位于染色體16p13.3，具有高度保守的3' UTR和串聯(lián)重復(fù)序列，其等位基因表現(xiàn)出序列變異，被認為是哮喘和其他過敏性和炎癥性疾病的發(fā)病機制中的介質(zhì)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎(chǔ)科研中肥大細胞相關(guān)機制的探索，如藥物干預(yù)實驗、疾病模型評估或炎癥因子網(wǎng)絡(luò)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Tpsab1 ELISA Kit; Mcp7 ELISA Kit; Mcpt7 ELISA Kit; Tpsb1 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; rMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit; Tryptase ELISA Kit; skin ELISA Kit
  • 縮寫：
    TPSAB1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, urine
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %90-92
    1:2Average %92
    Range %87-96
    1:4Average %94
    Range %89-97
    1:8Average %87
    Range %82-92
  • 回收率：
    The recovery of rat MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)104100-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.464 2.489 2.477 2.326
    201.823 1.863 1.843 1.692
    101.229 1.263 1.246 1.095
    50.812 0.847 0.830 0.679
    2.50.482 0.498 0.490 0.339
    1.250.312 0.339 0.326 0.175
    0.6250.241 0.258 0.250 0.099
    00.150 0.152 0.151 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
  • 亞細胞定位：
    Secreted. Note=Released from the secretory granules upon mast cell activation.
  • 蛋白家族：
    Peptidase S1 family, Tryptase subfamily
  • 組織特異性：
    Mast cells.
  • 數(shù)據(jù)庫鏈接：