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  4. Rat Membrane primary amine oxidase(AOC3) ELISA kit

Rat Membrane primary amine oxidase(AOC3) ELISA kit

  • 中文名稱(chēng)：
    大鼠伯胺氧化酶膜(AOC3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001855RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠伯胺氧化酶膜(AOC3)酶聯(lián)免疫試劑盒（CSB-EL001855RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的AOC3含量。AOC3又稱(chēng)血管粘附蛋白-1，是一種膜結(jié)合糖蛋白，在多種細(xì)胞中表達(dá)。與炎癥、免疫反應(yīng)等生理病理過(guò)程密切相關(guān)。研究機(jī)制方面，它能通過(guò)催化胺類(lèi)氧化影響細(xì)胞黏附、遷移等，在炎癥性疾病、心血管疾病等研究中有重要意義。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，適用于體外實(shí)驗(yàn)中對(duì)炎癥相關(guān)疾病模型、藥物干預(yù)效果或基因表達(dá)調(diào)控的科研分析，尤其適合探究AOC3在組織微環(huán)境中的動(dòng)態(tài)變化及其分子調(diào)控網(wǎng)絡(luò)，為炎癥機(jī)制研究、靶向藥物開(kāi)發(fā)等基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Aoc3; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VP97; Vascular adhesion protein 1; VAP-1
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %84-94
    1:2Average %96
    Range %91-102
    1:4Average %84
    Range %81-88
    1:8Average %93
    Range %88-99
  • 回收率：
    The recovery of rat AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9491-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.075 2.086 2.081 1.931
    2501.540 1.669 1.605 1.455
    1251.111 1.156 1.134 0.984
    62.50.758 0.750 0.754 0.604
    31.20.437 0.465 0.451 0.301
    15.60.261 0.277 0.269 0.119
    7.80.211 0.223 0.217 0.067
    00.149 0.150 0.150 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • 基因功能參考文獻(xiàn)：
    1. Data show that 35 potential amine oxidase copper containing 3 (SSAO)-interacting proteins were found to be altered in type 2 Diabetes (T2D). PMID: 28910971
    2. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
    3. The changes of K(M) and k(c)/K(M) values of copper amine oxidase appear due to the presence of positively charged groups in the substrate molecules. PMID: 20686801
    4. Provide evidence for a role for VAP-1 in the inflammatory outcome of diabetic retinopathy. Specifically, VAP-1 plays a central role in regulating leukocyte transmigration rate during chronic low-grade inflammation. PMID: 19635478
    5. Our parsimonious conclusion is that there is a relation between the risk factors of AD and vascular dementia (diabetes, oxidative stress and chronic inflammation) and SSAO activity, which may originate from the vessel wall. PMID: 16524643
    6. Enzymatic activity of the atypical endothelial adhesion molecule AOC3, and possibly that of other related oxidases, is crucial for leukocyte exit from the vessels in inflamed joints in vivo. PMID: 16947396
    7. It was concluded that hydroxylamine exerts hypotension partly through conversion to nitric oxide and partly by a "hydralazine-like" mechanism involving SSAO inhibition PMID: 17385063
    8. BZA + V treatment did not reduce HbA(1C) levels,but it reduced the elevated serum SSAO activity, decreased advanced-glycation end products and increased nitric oxide in diabetic animals. PMID: 17431735
    9. evidence for an important role of VAP-1 in the recruitment of macrophages to choroidal neovascularization lesions, establishing a novel link between VAP-1 and angiogenesis PMID: 18436961

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  • 亞細(xì)胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Copper/topaquinone oxidase family
  • 組織特異性：
    Highly expressed in adipocytes, aorta and lung. Expressed at lower levels in heart, kidney, large intestine, liver, small intestine and stomach.
  • 數(shù)據(jù)庫(kù)鏈接：