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  4. Rat Mucin-5 subtype AC(MUC5AC)ELISA Kit

Rat Mucin-5 subtype AC(MUC5AC)ELISA Kit

  • 中文名稱：
    大鼠粘蛋白/粘液素5AC(MUC5AC)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16223r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠粘蛋白/粘液素5AC(MUC5AC)酶聯(lián)免疫試劑盒（CSB-E16223r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MUC5AC含量。MUC5AC 是一種黏蛋白。它在呼吸道等部位發(fā)揮重要作用，其異常表達(dá)與哮喘、慢性阻塞性肺疾病等多種呼吸系統(tǒng)疾病密切相關(guān)。研究機(jī)制方面，主要聚焦于調(diào)控其表達(dá)、分泌的信號通路，以探索通過干預(yù)這些通路來治療相關(guān)疾病的方法。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，本試劑盒適用于基礎(chǔ)科研領(lǐng)域，例如研究呼吸道疾病模型（如哮喘、慢性阻塞性肺?。┲叙ひ悍置谡{(diào)控機(jī)制，評估胃腸道黏膜屏障功能變化，或探索藥物、環(huán)境因素對黏液代謝通路的影響，為相關(guān)生物標(biāo)志物研究及分子機(jī)制解析提供可靠工具。適用于實(shí)驗(yàn)室常規(guī)檢測及高通量篩查需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 94  
    Range % 87-97  
    1:10 Average % 97  
    Range % 92-102  
    1:20 Average % 92  
    Range % 88-97  
    1:40 Average % 95  
    Range % 89-100  
  • 回收率：
    The recovery of rat MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 85-95  
    EDTA plasma (n=4) 99 95-106  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.179 2.254 2.217 2.070  
    2000 1.614 1.607 1.611 1.464  
    1000 1.214 1.156 1.185 1.038  
    500 0.864 0.850 0.857 0.710  
    250 0.529 0.564 0.547 0.400  
    125 0.335 0.316 0.326 0.179  
    62.5 0.225 0.233 0.229 0.082  
    0 0.146 0.148 0.147 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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