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Rat Myeloperoxidase (MPO) ELISA kit

  • 中文名稱:
    大鼠髓過(guò)氧化物酶(MPO)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08722r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠髓過(guò)氧化物酶(MPO)酶聯(lián)免疫試劑盒(CSB-E08722r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的MPO含量。MPO(髓過(guò)氧化物酶)是一種血紅素蛋白酶,存在于中性粒細(xì)胞中,參與免疫和炎癥反應(yīng)。其研究背景涉及其在多種疾病中的作用,如心血管疾病和腫瘤。研究機(jī)制主要關(guān)注其基因表達(dá)、酶活性及其與宿主防御的相互作用。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL,該產(chǎn)品廣泛應(yīng)用于炎癥機(jī)制研究、動(dòng)脈粥樣硬化模型評(píng)估、神經(jīng)退行性疾病研究等科研領(lǐng)域,也可用于藥物干預(yù)實(shí)驗(yàn)中生物標(biāo)志物的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %94
    Range %90-98
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-102
  • 回收率:
    The recovery of rat MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9287-98
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.347 2.232 2.290 2.109
    101.416 1.386 1.401 1.220
    50.902 0.887 0.895 0.714
    2.50.602 0.612 0.607 0.426
    1.250.421 0.439 0.430 0.249
    0.6250.312 0.326 0.319 0.138
    0.3120.274 0.256 0.265 0.084
    00.182 0.180 0.181  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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