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  4. Rat Neuromodulin(GAP43) ELISA kit

Rat Neuromodulin(GAP43) ELISA kit

  • 中文名稱：
    大鼠神經(jīng)調(diào)制蛋白(GAP43)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL009231RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠神經(jīng)調(diào)制蛋白(GAP43)酶聯(lián)免疫試劑盒（CSB-EL009231RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、細(xì)胞裂解物、組織勻漿樣本中的GAP43含量。GAP43 即生長(zhǎng)相關(guān)蛋白 43，是神經(jīng)系統(tǒng)發(fā)育和再生過程中的重要蛋白。它參與軸突生長(zhǎng)、突觸可塑性等過程。研究發(fā)現(xiàn)，它在神經(jīng)損傷修復(fù)、學(xué)習(xí)記憶等機(jī)制中發(fā)揮作用，其表達(dá)變化與神經(jīng)系統(tǒng)疾病發(fā)展密切相關(guān)，有望成治療靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究，如神經(jīng)退行性疾病模型構(gòu)建、創(chuàng)傷后神經(jīng)再生機(jī)制探索以及體外神經(jīng)元培養(yǎng)實(shí)驗(yàn)中GAP43動(dòng)態(tài)變化的定量分析。為神經(jīng)系統(tǒng)相關(guān)疾病的分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Gap43 ELISA Kit; Neuromodulin ELISA Kit; Axonal membrane protein GAP-43 ELISA Kit; Growth-associated protein 43 ELISA Kit; Protein F1 ELISA Kit
  • 縮寫：
    GAP43
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell lysates, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GAP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %90-102
    1:2Average %102
    Range %98-108
    1:4Average %94
    Range %88-99
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of rat GAP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-102
    EDTA plasma (n=4)9490-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.107 2.276 2.192 2.037
    101.513 1.616 1.565 1.410
    50.955 0.923 0.939 0.784
    2.50.546 0.557 0.552 0.397
    1.250.389 0.372 0.381 0.226
    0.6250.271 0.286 0.279 0.124
    0.3120.191 0.198 0.195 0.040
    00.154 0.156 0.155
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
  • 基因功能參考文獻(xiàn)：
    1. miR-204 promoted the apoptosis of retinal cells through inhibiting GAP-43 in optic nerve injury. PMID: 29286154
    2. The data indicate that endogenous Nrn1 and Gap43 mRNAs compete for binding to HuD for their axonal localization and activity of the Nrn1 3'UTR. PMID: 28871047
    3. Dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats. PMID: 28801169
    4. Results show that repeated contingent, but not non-contingent, cocaine self-administration promotes an increase of nELAV expression immediately and 7 days after the last cocaine self-administration session, with a transient disappearance observed 24 h later. The evidence that the ubiquitous ELAV/HuR is not changed calls for a specific involvement of nELAV in these molecular mechanisms. PMID: 26850084
    5. Data show that growth-associated protein 43 (GAP43) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) transplantation can increase survival photoreceptor cells and delay retinal degeneration PMID: 27412933
    6. Data show that the paw skin and back skin revealed a significantly higher number of nerve fibres expressing growth-associated protein 43 (GAP-43) at both the acute and chronic stages of antigen-induced arthritis (AIA). PMID: 26503622
    7. astrocytic GAP43 upregulation may serve to indicate beneficial astrogliosis after central nervous system injury PMID: 26865625
    8. Lentiviral-mediated GAP-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats. PMID: 26238991
    9. Thus, unilateral deafness directly results in an asymmetrical adaptation of the gap43 transcription between both sides of the auditory brain stem. PMID: 25686598
    10. Phosphorylation at Ser-41 is required for plasma membrane association of GAP43. PMID: 25165142
    11. Studied whether melatonin would modulate the PSD-95, GAP-43, and MMP-9 proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to experimental stroke. PMID: 24350898
    12. The manifestation of clinical signs of experimental autoimmune encephalomyelitis has been found to be in correlation with the levels of GAP-43 proteolysis. PMID: 25868328
    13. Results show that PKC-dependent phosphorylation of GAP43 plays a critical role in regulating postsynaptic gephyrin aggregation in developing GABAergic synapses. PMID: 25755278
    14. Cocultivation with human adipose-derived mesenchymal stem cells restored GAP-43 expression and distribution in cortical neurons. PMID: 24458787
    15. There was no significant difference in immunohistochemical staining for transforming growth factor beta 3 between the control injured and obese injured groups PMID: 24665937
    16. KSRP modulation of GAP-43 mRNA stability restricts axonal outgrowth in embryonic hippocampal neurons. PMID: 24244461
    17. Progesterone promotes neuroprotection following traumatic brain injury by inhibiting the expression of Nogo-A and GFAP, and increasing GAP-43 expression. PMID: 24567055
    18. an AU-rich regulatory element (ARE) in GAP-43's 3'UTR is necessary and sufficient for axonal localization of Zbp1 PMID: 23586486
    19. Both GAP43 protein and mRNA levels were increased in dorsal root gangion neurons treated with neurotrophins and cocultured with skeletal muscle, implicating NTs and target skeletal muscle cells in DRG neuronal regeneration. PMID: 23666783
    20. Intradiscal inflammation might induce the expression of GAP-43, and potentially promote the nerve fiber ingrowth of rat. PMID: 23316631
    21. Ilexonin A markedly enhanced the neurogenesis in the brain after ischemia-reperfusion, and upregulated bFGF and GAP-43. PMID: 22121776
    22. Willed movement therapy increases the expression of NT-3 and GAP-43 in the ischemic brain area in rats with cerebral ischemia-reperfusion. PMID: 21868332
    23. siRNA-mediated depletion of overall GAP-43 mRNA from dorsal root ganglia decreases the length of axons. PMID: 23426659
    24. The mean brightness of GAP-43 per unit area of the of the dendritic tree does not vary across the estrous cycle; however, the modified brightness-area-product of GAP-43 changes significantly along the estrous cycle. PMID: 21948316
    25. Clonidine and dexmedetomidine treatment decreases the expression of GAP-43 in rat dorsal root ganglion during the development of chronic neuropathic pain. PMID: 22490962
    26. further evidence to support a key role for GAP-43 in brain development. PMID: 22617637
    27. IGF-1 may play an important role in neuroprotective effects on dorsal root ganglion neurons through regulating GAP-43 expression with excitotoxicity induced by Glu and the process was involved in both ERK1/2 and PI3K/Akt signaling pathways. PMID: 21822733
    28. Spinal GAP-43 is upregulated in the superficial L5 dorsal horn from 5 to 10 days after spinal cord injury; GAP-43/calcitonin-gene related peptide (CGRP) fibers may be indicative of sprouting peptidergic fibers. PMID: 21671799
    29. Noise-induced tinnitus is suppressed by strong up-regulation of GAP-43 in the medial ventral cochlear nucleus. PMID: 21821100
    30. This study suggested that GAP-43 liver change during function recovery after cerebellar damage. PMID: 22063813
    31. In PMCA-suppressed lines mRNA and protein levels of GAP43 were increased, however, the amount of phosphorylated form was lower than in control cells PMID: 21912933
    32. Exposure to low level lead during pregnancy could inhibit the GAP-43 protein and mRNA expression in the hippocampus of offspring. PMID: 18724891
    33. results show that the combination of repeated exposure to cocaine and acute stress significantly enhances nELAV expression and phosphorylation in the hippocampus with a concomitant increase of GAP43 expression PMID: 21210085
    34. BDNF and GAP43 may play an important role during the dynamic transhemispheric functional reorganization. PMID: 21723373
    35. GAP-43 is essential both to maintain climbing fibres structure in non-traumatic condition and to promote sprouting after partial lesion of the inferior olive. PMID: 21695168
    36. The expression of synaptophysin (SYP) and regenerationassociated protein (GAP43) in the injured brain of neural stem cells-transplanted rats was significantly increased. PMID: 21687946
    37. GFAP expression was down-regulated while GAP-43 expression upregulated in the retinal ganglial cells after peripheral nerve transplantation. PMID: 20423852
    38. Endogenous BDNF may regulate the expression of GAP-43 in the spinal cord anterior horn after sciatic nerve injury in rats. PMID: 20335141
    39. Significant correlations between gene and protein expressions were also observed for each transcript of BDNF, GAP-43, and NCAM1. PMID: 21436126
    40. Ttrahydroxystilbene glucoside can improve the neurological function through increasing the expressions of NGF and GAP-43 of cerebral ischemia-reperfusion rats. PMID: 21137370
    41. Data suggest that the expressions of GAP-43 mRNA and protein may be upregulated after brachial plexus injury, and GAP-43 protein is possibly associated with the axon regeneration and function reconstruction. PMID: 21167390
    42. results suggest that expression of GAP-43 in avulsed motoneurons is related to axonal regeneration whereas nNOS is not. PMID: 20667480
    43. Chondroitinase ABC can enhance the expression of GAP-43 after spinal cord injury. PMID: 21046809
    44. Study confirmed colocalization of autonomic nerve fiber markers with growth-associated protein 43 PMID: 20090303
    45. GAP-43 and P38 may be the pathological basis of insular epilepsy and the key molecular mechanism of synaptic plasticity. PMID: 20646586
    46. A role for pGAP-43 in retinotectal morphological plasticity observed both during normal development and after monocular enucleation is suggested. PMID: 20406666
    47. MMP-2, expressed and secreted by neurons and adjoining astrocytes, is a key actor in lesion-induced remodeling of central auditory networks and suggest a cooperativity with central axonal growth and synaptogenesis directed by GAP-43 PMID: 20173666
    48. study demonstrated that repeated maternal restraint stress increased GAP-43 in the postnatal rat brain during the second week of life but decreased after that PMID: 19782125
    49. Prenatal stress causes a significant increase of GAP-43 and pGAP-43 in the pup's hippocampus, which may alter the pattern of axonal growth and synapses' formation in the pup's brain PMID: 20035832
    50. Results provide marker proteins Gap-43 and newly identified candidate neuronal growth-associated proteins MAP1B, CRMP5, crmp5 and catenins. PMID: 19805073

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  • 亞細(xì)胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm.
  • 蛋白家族：
    Neuromodulin family
  • 組織特異性：
    Expressed in hippocampal neurons, with highest levels of expression in the CA4 and CA3 neurons and lower levels in CA1 neurons. Expressed in the dorsal root ganglion.
  • 數(shù)據(jù)庫鏈接：