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  4. Rat Neuron-specific enolase,NSE ELISA Kit

Rat Neuron-specific enolase,NSE ELISA Kit

  • 中文名稱：
    大鼠神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07963r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒（CSB-E07963r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ENO2含量。ENO2即烯醇化酶 2，在神經(jīng)組織等中高表達。它參與糖酵解過程，為細胞提供能量。異常表達與神經(jīng)母細胞瘤等腫瘤發(fā)生發(fā)展相關(guān)，可作為腫瘤診斷標志物與治療靶點，研究其機制有助于開發(fā)針對性治療策略。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于實驗動物模型（如腦損傷、神經(jīng)退行性疾?。┲蠳SE的動態(tài)監(jiān)測，或體外培養(yǎng)神經(jīng)細胞的功能研究，可支持多種樣本類型的批量檢測，為探索神經(jīng)系統(tǒng)疾病機制、評估神經(jīng)損傷程度或藥物干預(yù)效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %85-94
    1:2Average %100
    Range %97-104
    1:4Average %90
    Range %87-94
    1:8Average %100
    Range %95-103
  • 回收率：
    The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-98
    EDTA plasma (n=4)9592-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.260 2.232 2.246 2.078
    201.454 1.486 1.470 1.302
    100.920 0.928 0.924 0.756
    50.654 0.663 0.659 0.491
    2.50.403 0.398 0.401 0.233
    1.250.322 0.314 0.318 0.150
    0.6250.266 0.257 0.262 0.094
    00.170 0.166 0.168 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 亞細胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Enolase family
  • 組織特異性：
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 數(shù)據(jù)庫鏈接：