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  4. Rat OX-2 membrane glycoprotein(CD200) ELISA kit

Rat OX-2 membrane glycoprotein(CD200) ELISA kit

  • 中文名稱：
    大鼠OX-2膜糖蛋白(CD200)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL004895RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠OX-2膜糖蛋白(CD200)酶聯(lián)免疫試劑盒（CSB-EL004895RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CD200含量。CD200是一種重要靶點(diǎn)。其作為跨膜糖蛋白，廣泛表達(dá)于多種細(xì)胞表面。研究機(jī)制方面，CD200可與CD200R結(jié)合，調(diào)節(jié)免疫反應(yīng)，在腫瘤免疫逃逸、自身免疫病等方面有重要作用，目前是免疫治療等領(lǐng)域的研究熱點(diǎn)。試劑盒檢測(cè)范圍為47 pg/mL-3000 pg/mL，適用于科研領(lǐng)域中對(duì)免疫調(diào)節(jié)機(jī)制的基礎(chǔ)研究，如炎癥性疾病模型、神經(jīng)退行性疾病或腫瘤微環(huán)境中的免疫逃逸機(jī)制探索，也可用于評(píng)估實(shí)驗(yàn)動(dòng)物模型中CD200表達(dá)水平與特定病理過(guò)程的相關(guān)性。適用于實(shí)驗(yàn)室常規(guī)檢測(cè)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Cd200; Mox2; OX-2 membrane glycoprotein; MRC OX-2 antigen; CD antigen CD200
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-88
    1:2 Average % 90
    Range % 86-94
    1:4 Average % 95
    Range % 90-100
    1:8 Average % 92
    Range % 88-96
  • 回收率：
    The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 106 102-113
    EDTA plasma (n=4) 98 94-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.271 2.317 2.294 2.103
    1500 1.429 1.369 1.399 1.208
    750 0.928 0.984 0.956 0.765
    375 0.589 0.568 0.579 0.388
    187.5 0.396 0.372 0.384 0.193
    94 0.288 0.304 0.296 0.105
    47 0.240 0.231 0.236 0.045
    0 0.188 0.193 0.191 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 組織特異性：
    Found on the surface of neurons, thymocytes, B-cells and follicular dendritic cells.
  • 數(shù)據(jù)庫(kù)鏈接：