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Rat Osteoprotegerin,OPG ELISA KIT

  • 中文名稱:
    大鼠骨保護(hù)素(OPG)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E07404r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠骨保護(hù)素(OPG)酶聯(lián)免疫試劑盒(CSB-E07404r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的TNFRSF11B含量。TNFRSF11B是重要的骨代謝調(diào)節(jié)靶點(diǎn)。其背景在于參與破骨細(xì)胞分化、增殖等過(guò)程,對(duì)維持骨穩(wěn)態(tài)至關(guān)重要。研究機(jī)制主要圍繞其與配體相互作用,通過(guò)下游信號(hào)通路調(diào)控骨骼發(fā)育和重建,在骨質(zhì)疏松等骨相關(guān)疾病研究中受關(guān)注。試劑盒檢測(cè)范圍為0.78 ng/ml- 50 ng/ml,靈敏度為0.195 ng/ml。適用于大鼠模型中的骨代謝機(jī)制研究、骨質(zhì)疏松藥物干預(yù)實(shí)驗(yàn)或骨組織病理學(xué)分析等科研需求本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Tnfrsf11b ELISA Kit; Opg ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit; Osteoprotegerin ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.78 ng/ml-50 ng/ml
  • 靈敏度:
    0.195 ng/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-92

    1:2

    Average %

    99

    Range %

    91-105

    1:4

    Average %

    98

    Range %

    92-104

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of rat OPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    100

    94-108

    EDTA plasma (n=4)

    102

    97-109

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    50

    1.906

    2.034

    1.970

    1.796

    25

    1.113

    1.067

    1.090

    0.916

    12.5

    0.594

    0.628

    0.611

    0.437

    6.25

    0.395

    0.409

    0.402

    0.228

    3.12

    0.270

    0.281

    0.276

    0.102

    1.56

    0.235

    0.250

    0.242

    0.069

    0.78

    0.210

    0.214

    0.212

    0.038

    0

    0.172

    0.175

    0.174

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    TNFRSF11B(也稱為OPG)是一種可溶性的分泌性蛋白,主要作用是通過(guò)結(jié)合RANKL(受體活化因子配體)來(lái)調(diào)控骨吸收和破骨細(xì)胞的形成。最近的研究表明,TNFRSF11B還參與調(diào)控其他生理和病理過(guò)程,如心血管疾病、炎癥和腫瘤。
  • 功能:
    Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
  • 基因功能參考文獻(xiàn):
    1. Adipose-derived stem cell-released osteoprotegerin protects cardiomyocytes from reactive oxygen species-induced cell death. PMID: 28931423
    2. An upregulation of OPG was observed in the ovariectomized rats with streptozotocin-induced type 1 diabetes mellitus plus daily Zn supplementation group compared with the nonsupplemented and control groups. PMID: 28473060
    3. the canonical Wnt pathway inhibitor DKK1 blocked the osteogenesis effect and rescued the ratio of RANKL/OPG in periodontal ligament stem cells under force treatment for 1h. PMID: 27154288
    4. FGF21 inhibited VSMCs calcification via OPG/RANKL system, and through P38 andPI3K/AKT pathways. PMID: 28774557
    5. this study shows that flavonoids of Astragalus exert protective effects against adjuvant arthritis by regulating OPG/RANKL/NF-kappaB pathway PMID: 28092862
    6. In addition, curcumin upregulated the mRNA expression levels of transcription factors that favor osteoblast differentiation and increased the ratio of OPG to RANKL. PMID: 26677102
    7. The ratio of RANKL to OPG was very low during most of the arterial calcification period, which allowed for OPG to completely inhibit RANKL-induced osteoclast-like cell differentiation and to promote calcification. PMID: 26489619
    8. RANKL and OPG expressions were not changed by chronic exposure to fluoride in the drinking water during the studied periods; on the other hand, TRAP expression was changed (at 7 days) by chronic exposure to fluoride PMID: 22878484
    9. Lowering the serum homocysteine level by a simultaneous supplementation with N-acetylcysteine improved OPG and FOXO1 expression and partially antagonized RANKL and proresorptive cytokine synthesis in the bone milieu PMID: 23500899
    10. High-dose diosgenin treatment up-regulated expression of OPG significantly in tibia from OVX rats compared to control. PMID: 25257532
    11. Data indicate that the highest expression level of osteoprotegerin(OPG) was observed in jump (J) group. PMID: 25200151
    12. Cadmium could enhance receptor-activated nuclear factor kappa B ligand expression (RANKL) and inhibit osteoprotegerin (OPG) expression. PMID: 23954550
    13. RANKL and OPG expression promotes osteoclast development and enhances its function under the condition of estrogen deficiency PMID: 24321069
    14. Pulsed electromagnetic field can prevent ovariectomy-induced bone loss through regulating the expression of RANKL and OPG. PMID: 22948539
    15. Bisphosphonate in bone cement affects bone turnover by stimulating an increase in osteoprotegerin and a decrease in RANKL in the bone microenvironment. PMID: 23404413
    16. The concentration of sRANKL in gingival crevicular fluid increased significatly, then decreased sharply after orthodontic retention and bone remodeling. PMID: 22353912
    17. Epimedium extract can prevent glucocorticoid-induced femoral head necrosis probably by antagonizing glucocorticiod-induced abnormal expressions of OPG and RANKL mRNA. PMID: 22027774
    18. Ultrasound stimulation might be useful to protect against root resorption and accelerate its repair by regulating the expressions of OPG and RANKL. PMID: 21602131
    19. Ovariectomy caused a significant decrease in OPG gene expression in the tibia and aorta PMID: 21519319
    20. The temporospatial expression patterns of osteoprotegerin and RANKL suggest that osteoblasts and the lineage cell network orchestrates bone remodelling during distraction osteogenesis. PMID: 18476557
    21. except for the period of time in tooth eruption, where osteoclast formation is needed to form an eruption pathway, secretion of OPG (osteoprotegerin) prevents resorption of alveolar bone and subsequent disruption of the periodontal ligament PMID: 11839361
    22. reduces osteoclast numbers and prevents bone erosion in collagen-induced arthritis PMID: 12368214
    23. Detection of osteoprotegerin mRNA and protein in bone cells. PMID: 15704000
    24. Regulation of OPG production by the dental follicle likely affects the alveolar bone resorption needed for tooth eruption. PMID: 16283633
    25. RANK expression stimulates the activation and differentiation of osteoclast-like cells via low level laser irradiation in osteoclast precursor cells. PMID: 16568210
    26. Cytokine-induced production of OPG may protect beta cells from further damage, an effect mediated through inhibition of p38 map kinase phosphorylation. PMID: 17443309
    27. Results describe the effects of the administration of corticosterone, parathyroid hormone, or both, and of their withdrawal, on rat bone and cartilage histomorphometric parameters, and on osteoprotegerin and RANKL mRNA expression and proteins. PMID: 17476578
    28. Systemic delivery of osteoprotegerin inhibits alveolar bone resorption in experimental periodontitis, suggesting that RANKL inhibition may represent an important therapeutic strategy for the prevention of progressive alveolar bone loss. PMID: 17608585
    29. abnormal elevation of the OPG/tumor necrosis factor (ligand) superfamily member 10 ratio in the vessel wall characterizes the early onset of diabetes mellitus PMID: 17627577
    30. Data demonstrate that hyperprolactinemia can act directly on bone to stimulate bone turnover, and that prolactin enhances bone resorption in part by increasing RANKL and decreasing osteoprotegerin expression by osteoblasts. PMID: 18166509
    31. OPG/RANK/RANKL axis may be involved in cardiac remodeling in immuno-inflammatory myocardial diseases and progression of chronic HF and thus may represent targets for intervention in this disorder. PMID: 18417124
    32. Prolactin decreased osteoprotegerin mrna in cultured osteoblasts. PMID: 18432284
    33. demonstrate an imbalance of RANKL/OPG expression associated with diabetes that may contribute to the delay of fracture repair during the course of diabetes PMID: 18592139
    34. HLA-B27 transgenic rats show a reduced bone mass, and suggest an involvement of the RANKL/OPG system in the mechanism of bone loss in this disease PMID: 19040304
    35. OPG is a positive regulator of microvessel formation, while RANKL is an angiogenic inhibitor due to effects on regulation of endothelial cell proliferation, apoptosis, and signaling. PMID: 19105036
    36. low bone turnover after 1 yr of OPG overexpression in rats was associated with increased vertebral bone mass and proportional increases in bone strength, with no evidence for deleterious effects on vertebral material properties. PMID: 19257823
    37. Different values of traction force cause a variation of the RANKL/OPG ratio through the expression of RANKL protein, modulating the activities of bone remodeling in the subchondral bone of condyle. PMID: 19537860
    38. Orchiectomized rats exhibited increased RANKL protein in bone marrow plasma and in bone marrow cells, with no changes in serum RANKL. PMID: 19539794

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  • 亞細(xì)胞定位:
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接: