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  4. Rat Pancreatic Lipase,PL ELISA Kit

Rat Pancreatic Lipase,PL ELISA Kit

  • 中文名稱：
    大鼠胰脂肪酶(PL)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13784r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰脂肪酶(PL)酶聯(lián)免疫試劑盒（CSB-E13784r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PNLIP含量。PNLIP即胰腺脂肪酶，是參與脂肪消化的關(guān)鍵酶。其背景在于它在人體脂肪代謝中起重要作用。研究機制主要圍繞其參與甘油三酯水解過程，通過特異性作用位點將甘油三酯分解為脂肪酸和甘油，以促進脂肪吸收，對其研究有助于探索脂肪代謝相關(guān)疾病。試劑盒檢測范圍為0.312 U/mL-20 U/mL，可滿足科研領(lǐng)域?qū)嶒瀯游锬Ｐ鸵认俟δ茉u估、脂肪代謝相關(guān)疾病機制研究的需求，例如在胰腺炎模型、肥胖代謝綜合征或藥物干預(yù)實驗中監(jiān)測胰脂肪酶動態(tài)變化，為營養(yǎng)學(xué)、藥理學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供穩(wěn)定可靠的分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Pnlip ELISA Kit; Pancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.312 U/mL-20 U/mL
  • 靈敏度：
    0.078 U/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 106
    Range % 103-109
    1:2 Average % 96
    Range % 91-101
    1:4 Average % 92
    Range % 87-97
    1:8 Average % 95
    Range % 89-99
  • 回收率：
    The recovery of rat PL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 96-106
    EDTA plasma (n=4) 94 87-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    U/ml OD1 OD2 Average Corrected
    20 2.267 2.355 2.311 2.181
    10 1.848 1.855 1.852 1.722
    5 1.230 1.262 1.246 1.116
    2.5 0.712 0.744 0.728 0.598
    1.25 0.374 0.355 0.365 0.235
    0.625 0.289 0.287 0.288 0.158
    0.312 0.222 0.212 0.217 0.087
    0 0.126 0.133 0.130 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
  • 基因功能參考文獻：
    1. Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat. PMID: 21469501
    2. Regulated expression of pancreatic triglyceride lipase after rat traumatic brain injury PMID: 19760487
    3. Data show that pancreatic lipase and pancreatic lipase related protein 2 gene expression is regulated anticoordinately by the amount of dietary polyunsaturated fat starting as early as the preweanling phase of development. PMID: 15181189
    4. Pnlip is possible candidate for the Nidd6/of QTL in the OLETF rat PMID: 15883013
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Expressed by the pancreas. Pancreatic secretory (zymogen) granule.
  • 數(shù)據(jù)庫鏈接：