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  4. Rat Pancreatic alpha-amylase(AMY2A) ELISA kit

Rat Pancreatic alpha-amylase(AMY2A) ELISA kit

  • 中文名稱：
    大鼠胰淀粉酶α2A(AMY2A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001689RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰淀粉酶α2A(AMY2A)酶聯(lián)免疫試劑盒（CSB-EL001689RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AMY2A含量。AMY2A是一個(gè)靶點(diǎn)。它主要編碼胰腺α -淀粉酶，在碳水化合物消化中起著關(guān)鍵作用。目前研究圍繞其與碳水化合物代謝相關(guān)機(jī)制展開，探究其功能異常與代謝疾病如糖尿病等的關(guān)聯(lián)，期望通過調(diào)節(jié)該靶點(diǎn)來改善相關(guān)病癥。試劑盒檢測范圍為3.12 mIU/mL-200 mIU/mL，該產(chǎn)品適用于科研領(lǐng)域中對大鼠胰腺功能的基礎(chǔ)研究，如評估營養(yǎng)代謝干預(yù)、胰腺炎模型或糖尿病等代謝性疾病動物模型的病理機(jī)制，也可用于藥物開發(fā)中胰腺相關(guān)生物標(biāo)志物的動態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    AMY2A
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 mIU/mL-200 mIU/mL
  • 靈敏度：
    0.78 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 95-104  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 99  
    Range % 94-105  
  • 回收率：
    The recovery of rat AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-101  
    EDTA plasma (n=4) 92 88-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    200 2.627 2.574 2.601 2.497  
    100 1.985 1.852 1.919 1.815  
    50 1.104 1.126 1.115 1.011  
    25 0.628 0.609 0.619 0.515  
    12.5 0.360 0.381 0.371 0.267  
    6.25 0.259 0.247 0.253 0.149  
    3.12 0.181 0.174 0.178 0.074  
    0 0.103 0.105 0.104    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Comparative proteomic analysis revealed that amylase levels were decreased by twofold in pancreases from hyperlipidemic rats compared with control rats.Pancreatic amylase protein levels were also decreased in tissue from hyperlipidemic rats. PMID: 20981735
    2. analysis of how amylase secretion is proteoglycan-dependent in rat parotid acinar cells PMID: 17980695
  • 亞細(xì)胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Glycosyl hydrolase 13 family
  • 數(shù)據(jù)庫鏈接：