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  4. Rat Paraoxonase 1 (PON1)ELISA Kit

Rat Paraoxonase 1 (PON1)ELISA Kit

  • 中文名稱：
    大鼠對氧磷酶-1(PON1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14340r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠對氧磷酶-1(PON1)酶聯(lián)免疫試劑盒（CSB-E14340r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PON1含量。PON1（對氧磷酶1）是一種與高密度脂蛋白（HDL）結(jié)合的水解酶，具有抗氧化和代謝有機(jī)磷農(nóng)藥的功能。其研究機(jī)制涉及PON1在有機(jī)磷農(nóng)藥代謝中的作用，以及與心血管疾病和動脈硬化的關(guān)系。PON1的基因多態(tài)性影響其活性，進(jìn)而影響個體對有機(jī)磷農(nóng)藥毒性和心血管疾病的風(fēng)險。試劑盒檢測范圍為15.6 μU/mL-1000 μU/mL，適用于多種科研場景下PON1水平的精準(zhǔn)分析，適用于毒理學(xué)研究、氧化應(yīng)激模型評估及代謝相關(guān)疾病的分子機(jī)制探索，為心血管、肝臟功能及環(huán)境毒物暴露等領(lǐng)域的科學(xué)研究提供高效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Pon1 ELISA Kit; Pon ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit; PON 1 ELISA Kit; EC 3.1.1.2 ELISA Kit; EC 3.1.1.81 ELISA Kit; EC 3.1.8.1 ELISA Kit; Aromatic esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit
  • 縮寫：
    PON1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 μU/mL-1000 μU/mL
  • 靈敏度：
    3.9 μU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %91
    Range %86-95
    1:400Average %102
    Range %97-107
    1:800Average %91
    Range %85-97
    1:1600Average %97
    Range %91-103
  • 回收率：
    The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9790-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/mlOD1OD2AverageCorrected
    10002.733 2.799 2.766 2.616
    5002.274 2.220 2.247 2.097
    2501.718 1.708 1.713 1.563
    1251.103 1.123 1.113 0.963
    62.50.641 0.682 0.662 0.512
    31.20.351 0.362 0.357 0.207
    15.60.245 0.236 0.241 0.091
    00.150 0.149 0.150
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
  • 基因功能參考文獻(xiàn)：
    1. Compared with the rats in the control group, the serum activity of xanthine oxidase was significantly higher and that of PON1 was significantly lower in the rats in the model nonalcoholic fatty liver disease group (P<0.01). PMID: 27959408
    2. Acai extract increased serum PON1 activity and reduced hepatic steatosis and hepatic injury in rats fed a high fat diet. PMID: 27642496
    3. investigated the changes in plasma PON1 activity, LDL oxidation, radical scavenging activity and lipid peroxidation in D-galactose-induced aging model and also compared the results with 24-month naturally aged rats PMID: 24214524
    4. Study documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training. PMID: 19091700
    5. High omega-3 PUFA and ethanol decreased liver PON1 mRNA expression. Betaine restored liver PON1 mRNA expressions in low and high omega-3 PUFA ETOH groups. PMID: 20028357
    6. increased N-homocysteinylation of plasma proteins in chronic hyperleptinemia caused by impairment of the animal's ability to hydrolyze homocysteine-thiolactone by PON1 PMID: 19887391
    7. increased PON1 level may have a function related to the well-known atherosclerosis resistance of rats PMID: 16052486
    8. caloric restriction reduced serum Paraoxonase 1 (PON1) activity and showed gender-related differences that are indicative of a different adaptive strategy of male and female rats when faced with a period of food restriction PMID: 16214326
    9. Result suggests that PON1 modulation is not an integral part of the main antioxidant mechanisms of caloric restriction. PMID: 16816326
    10. Serum PON1 activity increases within the first hours of fasting, representing a prompt adaptation designed to attenuate blood lipid peroxidation that cannot be sustained when fasting is prolonged PMID: 17146679
    11. Delivery of the PON-1 gene may be a possible therapeutic strategy for preventing atherosclerosis. PMID: 17460375
    12. Consumption of pistachio as 20% of daily caloric intake increased serum PON1 activity by 35%. PMID: 17464102
    13. Serum paraoxonase activities were increased in a dose-dependent manner in taurine-treated hypothyroid rats. PMID: 17645625
    14. Reduction in PON1 activity during Nippostrongylusbrasiliensis infection may be associated with inflammatory reactions. mounted against the parasites. PMID: 18458312
    15. Report gender related differences in paraoxonase 1 response to high-fat diet-induced oxidative stress. PMID: 18719679
    16. suggest a hepato-protective role for PON1 against inflammation, fibrosis and liver disease mediated by MCP-1. PMID: 19144177

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  • 亞細(xì)胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Paraoxonase family
  • 組織特異性：
    Plasma. Associated with HDL.
  • 數(shù)據(jù)庫鏈接：