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  4. Rat Pentosidine ELISA Kit

Rat Pentosidine ELISA Kit

  • 中文名稱：
    大鼠戊糖素(Pentosidine)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09416r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠戊糖素(Pentosidine)酶聯(lián)免疫試劑盒（CSB-E09416r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Pentosidine含量。Pentosidine是一種晚期糖基化終末產(chǎn)物（AGEs）。它與多種疾病相關(guān)，如糖尿病并發(fā)癥、心血管疾病等。其研究機制主要聚焦在它可介導氧化應激和炎癥反應，促進細胞功能障礙和組織損傷，是研究疾病發(fā)病機制及尋找潛在治療靶點的重要方向。試劑盒檢測范圍為15.6 pmol/mL-1000 pmol/mL，在代謝性疾病機制、氧化應激研究及抗衰老藥物開發(fā)等科研領域具有重要價值；尤其適用于糖尿病模型大鼠的血清/血漿樣本分析，或肝臟、腎臟等組織的勻漿液檢測；為研究戊糖素在慢性疾病發(fā)展中的作用機制、評估干預措施效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Pentosidine
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pmol/mL-1000 pmol/mL
  • 靈敏度：
    3.9 pmol/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-89
    1:2Average %89
    Range %85-93
    1:4Average %96
    Range %92-100
    1:8Average %92
    Range %86-97
  • 回收率：
    The recovery of rat Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10196-105
    EDTA plasma (n=4)9085-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    10002.065 2.051 2.058 1.966
    5001.361 1.292 1.327 1.235
    2500.745 0.697 0.721 0.629
    1250.389 0.412 0.401 0.309
    62.50.219 0.233 0.226 0.134
    31.20.189 0.201 0.195 0.103
    15.60.127 0.138 0.133 0.041
    00.094 0.089 0.092 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

Regarding your ELISA kits CSB-E09416r, could you please advise on the following:
1) Are the standards natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Do the standards include additive agents such as antiseptic or preservative? If they include, What is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry.
CSB-E09416r Standard is purified Pentosidine polymer.
Preservative is porclin 300(1:3000).
Stop solution is 4N sulfuric acid；
These kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.